用于在临床实验室中鉴定为产酸克雷伯菌的菌株中检测拉乌尔菌属分离株的肠杆菌重复基因间共有序列1R聚合酶链反应检测法
Enterobacterial repetitive intergenic consensus 1R PCR assay for detection of Raoultella sp. isolates among strains identified as Klebsiella oxytoca in the clinical laboratory.
作者信息
Granier Sophie A, Leflon-Guibout Véronique, Goldstein Fred W, Nicolas-Chanoine Marie-Hélène
机构信息
Service de Microbiologie-Hygiène, Hôpital Ambroise Paré AP-HP, Boulogne, France.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1740-2. doi: 10.1128/JCM.41.4.1740-1742.2003.
Abstract
The enterobacterial repetitive intergenic consensus 1R PCR method, which provided recognizable profiles for reference strains of the three species of Raoultella and the two genetic groups of Klebsiella oxytoca, was applied to 19 clinical isolates identified as K. oxytoca. By this method, as confirmed by species-specific gene sequencing, two Raoultella ornithinolytica and two unclassifiable K. oxytoca isolates were identified.
摘要
肠杆菌重复基因间共有序列1R PCR方法可为三种拉乌尔菌属参考菌株以及产酸克雷伯菌的两个基因组提供可识别的图谱，该方法被应用于19株鉴定为产酸克雷伯菌的临床分离株。通过这种方法，并经种特异性基因测序确认，鉴定出两株解鸟氨酸拉乌尔菌和两株无法分类的产酸克雷伯菌分离株。
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