人消化道黏膜主要神经节苷脂的甲基化分析
Methylation analysis of the major gangliosides of the human alimentary mucosa.
作者信息
Keranen A
出版信息
Biochim Biophys Acta. 1976 Apr 22;431(1):96-104. doi: 10.1016/0005-2760(76)90263-0.
PMID:1268248
Abstract
The five major gangliosides of the human alimentary mucosa were purified with silicic acid column chromatography and with thin-layer chromatography. The linkages in the carbohydrate portion were analysed by permethylation with gas chromatography-mass spectrometry. A succesful analysis of the linkages of two hematosides and three tetraglycosylceramides was performed. Two of the tetraglycosylamides had a galactosamine in their chain and one had a glucosamine.
摘要
人消化道黏膜的五种主要神经节苷脂通过硅酸柱色谱法和薄层色谱法进行了纯化。碳水化合物部分的连接通过气相色谱 - 质谱联用的全甲基化法进行分析。成功完成了两种血苷和三种四糖基神经酰胺连接的分析。其中两种四糖基酰胺在其链中有一个半乳糖胺，一种有一个葡萄糖胺。
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