Zheng Yu-fang, Chen Ying-jie, Pang Tao, Shi Xian-zhe, Kong Hong-wei, Lü Shen, Yang Qing, Xu Guo-wang
National Chromatographic R. & A. Center, Dalian Insuitute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
Se Pu. 2002 Nov;20(6):498-501.
A method for the determination of urinary nucleosides with reversed-phase high performance liquid chromatography is described. After nucleosides were extracted from urine on phenyl boronic acid affinity chromatography, the analysis was performed on a column (4.6 mm i.d. x 250 mm, 5 microns) at 22 degrees C using a linear gradient elution comprising 25 mmol/L KH2PO4 solution (pH 4.55) and 60% methanol in water with UV detection at 260 nm. This method was used for the determination of 15 urinary nucleosides of 41 intestinal cancer patients and 52 normal adults. The results showed that the average concentrations of 12 urinary nucleosides from intestinal cancer patients were much higher than those of normal adults with P < 0.001. Using the concentrations of 15 nucleosides as the data vectors, principal component analysis was applied to classify intestinal cancer patients and normal adults and 76% (31/41) of the cancer patients were correctly classified. It is concluded that the method is sensitive, reliable and suitable for basic research and clinical applications to malignant tumours.
描述了一种用反相高效液相色谱法测定尿核苷的方法。尿核苷在苯基硼酸亲和色谱上从尿液中提取后，在22℃下于内径4.6mm×250mm、5μm的色谱柱上进行分析，使用由25mmol/L KH2PO4溶液（pH 4.55）和60%甲醇水溶液组成的线性梯度洗脱，并在260nm处进行紫外检测。该方法用于测定41例肠道癌患者和52例正常成年人的15种尿核苷。结果显示，肠道癌患者12种尿核苷的平均浓度显著高于正常成年人，P<0.001。以15种核苷的浓度作为数据向量，应用主成分分析对肠道癌患者和正常成年人进行分类，76%（31/41）的癌症患者被正确分类。结论是该方法灵敏、可靠，适用于恶性肿瘤的基础研究和临床应用。
