Xu G, Di Stefano C, Liebich H M, Zhang Y, Lu P
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences.
J Chromatogr B Biomed Sci Appl. 1999 Sep 24;732(2):307-13. doi: 10.1016/s0378-4347(99)00296-0.
Post-transcriptional modifications in RNA give rise to free modified ribonucleosides circulating in the blood stream and excreted in urine. Due to their abnormal levels in conjunction with several tumor diseases, they have been suggested as possible tumor markers. The developed RP-HPLC method has been applied to analyze the urinary nucleosides in 34 urinary samples from 15 kinds of cancer patients. The statistical analyses showed the urinary nucleoside excretion, especially modified nucleoside levels, in cancer patients were significantly higher than those in normal healthy volunteers. Factor analysis was used to classify the patients with cancer and normal healthy humans. It was found that using 15 urinary nucleoside levels or only five modified nucleoside levels as data vectors the factor analysis plot displayed two almost separate clusters representing each group.
RNA的转录后修饰会产生游离的修饰核糖核苷，它们在血流中循环并随尿液排出。由于它们在几种肿瘤疾病中的异常水平，它们被认为可能是肿瘤标志物。所开发的反相高效液相色谱（RP-HPLC）方法已应用于分析来自15种癌症患者的34份尿液样本中的尿核苷。统计分析表明，癌症患者的尿核苷排泄，尤其是修饰核苷水平，显著高于正常健康志愿者。因子分析用于对癌症患者和正常健康人进行分类。结果发现，以15种尿核苷水平或仅5种修饰核苷水平作为数据向量时，因子分析图显示出两个几乎分开的簇，分别代表每组。
