[高效毛细管电泳法测定白头翁汤中黄连与黄柏合煎液中的生物碱]

[Determination of alkaloids from Coptis chinensis Franch. and Phellodendron amurense Rupr. Decocted together in baitouweng decocta by high performance capillary electrophoresis].

作者信息

Wei Pei-hai, Chen Li-ren, Wei Ying-qin, Yuan Jiu-rong, Ma Ji-ping, Chen Ling-xin

机构信息

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730020, China.

出版信息

Se Pu. 2002 Nov;20(6):554-6.

PMID:12683007

Abstract

A rapid and accurate method for determining alkaloids from Coptis chinensis Franch, and Phellodendron amurense Rupr, decocted together in Baitouweng decocta was established. Experiments were carried out on a self-assembled capillary electrophoresis system. The experimental conditions were as follows: 75 microns i.d. x 50 cm fused silica capillary, 0.05 mol/L Na2B4O7-CH3OH (85:15, V/V) as buffer, applied voltage 14 kV and detection wavelength 232 nm. In addition, the concentration of ethanol in the extraction solvent was optimized. Experimental results showed that ethanol-water (30:70, V/V) was the ideal solvent to extract the main effective ingredients from Coptis chinensis Franch. and Phellodendron amurense Rupr. decocted together in Baitouweng decocta. Berberine and palmatine had good linearities in the range of 15.0 mg/L-65.0 mg/L and 12.5 mg/L-50.0 mg/L respectively, and their average recoveries were 95.5%-104% (RSD 2.7%-6.9%) and 92.1%-103% (RSD 4.5%-6.7%) respectively.

摘要

建立了一种快速准确测定白头翁汤中黄连和黄柏生物碱的方法。实验在自行组装的毛细管电泳系统上进行。实验条件如下：内径75微米×50厘米熔融石英毛细管，以0.05 mol/L硼砂-甲醇（85:15，V/V）为缓冲液，施加电压14 kV，检测波长232 nm。此外，对提取溶剂中乙醇的浓度进行了优化。实验结果表明，乙醇-水（30:70，V/V）是从白头翁汤中提取黄连和黄柏主要有效成分的理想溶剂。小檗碱和巴马汀分别在15.0 mg/L - 65.0 mg/L和12.5 mg/L - 50.0 mg/L范围内具有良好的线性关系，其平均回收率分别为95.5% - 104%（RSD 2.7% - 6.9%）和92.1% - 103%（RSD 4.5% - 6.7%）。