反相高效液相色谱法测定枳实和枳壳中橙皮苷和柚皮苷的含量

[Determination of hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantii by reversed-phase high performance liquid chromatography].

作者信息

Li Xiu-ling, Li Long, Xiao Hong-bin, Liang Xin-miao

机构信息

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.

出版信息

Se Pu. 2002 Nov;20(6):585-6.

PMID:12683015

Abstract

An HPLC method was established for the determination of hesperidin and naringin in the Fructus Aurantii Immaturus and Fructus Aurantii from different places. Hesperidin and narigin were separated on a Hypersil ODS1 column with acetonitrile-0.5% acetic acid (volume ratio of 22:78) as the mobile phase and detected at 283 nm. The flow rate was 1.0 mL/min. The column temperature was set at 35 degrees C. The calibration curves were linear in the ranges of 0.060 g/L-0.604 g/L and 0.0125 g/L-0.125 g/L for naringin and hesperidin respectively. The average recoveries were 97.1% for naringin and 95.3% for hesperidin. The method is simple, rapid, accurate and reliable.

摘要

建立了一种高效液相色谱法用于测定不同产地枳实和枳壳中的橙皮苷和柚皮苷。橙皮苷和柚皮苷在Hypersil ODS1柱上分离，以乙腈 - 0.5%乙酸（体积比22:78）为流动相，在283 nm波长处检测。流速为1.0 mL/min。柱温设定为35℃。柚皮苷和橙皮苷的校准曲线分别在0.060 g/L - 0.604 g/L和0.0125 g/L - 0.125 g/L范围内呈线性。柚皮苷的平均回收率为97.1%，橙皮苷的平均回收率为95.3%。该方法简便、快速、准确、可靠。