Cao Jun, Gong Qian-Feng, Xiao Bing-Hua, Wang Shao-Jun, Wang Yue-Sheng, Yang Shi-Lin
Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(4):301-3.
To establish a method for HPLC fingerprint determination of different growing areas on Fructus Aurantii fried with bran.
HPLC chromatographic experiment was carried out by ultrasonic distilment of acetacetic ester, gradient elution mode and the detection wavelength of 320 nm. The column temperature was set at 30 degrees C, and flow rate is 1.0 mL x min(-1). Naringin served as the reference standard. Ten batches of Fructus Aurantii fried with bran in the different places were analyzed. Similarity evaluation system for chromatographic fingerprint of TCM proceeded the evaluation, The correlation coefficients between ten batches were used to define the similarity.
There were eleven characteristic peaks in Fructus Aurantii fried with bran by HPLC fingerprint which constituted fingerprint characters. Both the reproducibility and stability on HPLC fingerprint were proper.
The method was accurate and simple. It can be applied to the analysis on Fructus Aurantii fried with bran. The fingerprint spectrum can be used to distinguish Fructus Aurantii fried with bran and to be its quality control.
建立麸炒枳壳不同产地的高效液相色谱指纹图谱测定方法。
采用乙酸乙酯超声提取，梯度洗脱，检测波长320nm进行高效液相色谱实验。柱温设定为30℃，流速为1.0mL·min⁻¹。以柚皮苷为对照品。对10批不同产地的麸炒枳壳进行分析。采用中药色谱指纹图谱相似度评价系统进行评价，以10批样品的相关系数确定相似度。
麸炒枳壳高效液相色谱指纹图谱中有11个特征峰，构成指纹特征。高效液相色谱指纹图谱的重复性和稳定性良好。
该方法准确、简便，可用于麸炒枳壳的分析。指纹图谱可用于鉴别麸炒枳壳并作为其质量控制。
