枳实类方剂颗粒汤剂中三种主要黄酮类化合物的超高效液相色谱同步分析。
Simultaneous UPLC analysis of three major flavonoids in granule decoctions of Fructus aurantii-type formulae.
作者信息
Huang Wei, Xiong Zhi-Hua, Huang Xi, Chen Xiao, Liu Wei-Ping, Wang Yang, Ren Ping
机构信息
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Ganzhou, China.
出版信息
Pharmazie. 2012 Jul;67(7):586-9.
A simple, rapid, and sensitive liquid chromatographic method has been established for the simultaneous analysis of three compounds (narirutin, hesperidin and naringin), in granule decoctions of Fructus Aurantii-type formulae. The compounds were separated in less than 10 min using a C18 column with gradient elution using (A) acetonitrile, (B) water, and (C) acetic acid at a flow rate of 0.3 mL/min, and with a PDA detector. The method was validated for specificity, accuracy, precision, and limits of detection. Good linear regression data (r2 > 0.9980) were obtained for all the calibration plots within the ranges tested. The method is an attractive alternative for quality control and clinical monitor of granule decoctions of Fructus Aurantii-type formulae.
已建立一种简单、快速且灵敏的液相色谱法，用于同时分析枳壳类方剂颗粒汤剂中的三种化合物（橙皮芸香苷、橙皮苷和柚皮苷）。使用C18柱，以0.3 mL/min的流速，采用（A）乙腈、（B）水和（C）乙酸进行梯度洗脱，并使用PDA检测器，在不到10分钟内分离出这些化合物。该方法在特异性、准确性、精密度和检测限方面得到了验证。在所测试的范围内，所有校准曲线均获得了良好的线性回归数据（r2 > 0.9980）。该方法是枳壳类方剂颗粒汤剂质量控制和临床监测的一种有吸引力的替代方法。
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