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[淋巴细胞群体中的细胞组成与辐射适应性反应]

[The cell composition in the lymphocyte population and the radiation adaptive response].

作者信息

Serebrianyĭ A M, Aleshchenko A V, Gotlib V Ia, Kudriashova O V, Semenova L P, Pelevina I I

机构信息

Institute of Biochemical Physics RAS, Moscow.

出版信息

Tsitologiia. 2003;45(1):81-5.

Abstract

Using lymphocytes of 5 healthy individuals the ability to adaptive response (AR), cell composition of population after PHA stimulation, changes in cell composition population after irradiation in the dose of 1.0 Gy and after irradiation in adaptive (0.05 Gy) and challenge (1.0 Gy) doses have been studied. AR observed in 2 of the 5 individuals only. After PHA stimulation the persons with AR have the total amount of cells after mitosis or during mitosis (the number of binucleated cells + the number of multinucleated cells + the whole cells with micronuclei + the number of mitotic cells) on average is higher than in persons without AR. In individuals with AR the linear correlation between the number of binucleated cells with micronuclei (on the 1000 scored binucleated cells) and the part of binucleated cells in the population is observed with coefficients of correlation -0.89 and -0.91. In the humans without AR this correlation is absent. The correlation observed permits to suppose that AR may occur at the expense of not only the decrease in number of damaged lymphocytes, but also the increase in the share of not damaged binucleated cell with the stable number of damaged cells.

摘要

利用5名健康个体的淋巴细胞,研究了适应性反应(AR)能力、PHA刺激后群体的细胞组成、1.0 Gy剂量照射后以及适应性(0.05 Gy)和激发(1.0 Gy)剂量照射后群体细胞组成的变化。仅在5名个体中的2名观察到AR。PHA刺激后,有AR的人有丝分裂后或有丝分裂期间的细胞总数(双核细胞数+多核细胞数+有微核的全细胞数+有丝分裂细胞数)平均高于无AR的人。在有AR的个体中,观察到有微核的双核细胞数(在1000个计分的双核细胞中)与群体中双核细胞部分之间的线性相关性,相关系数为-0.89和-0.91。在无AR的人中不存在这种相关性。观察到的相关性表明,AR的发生可能不仅是以受损淋巴细胞数量的减少为代价,而且是以未受损双核细胞比例的增加为代价,而受损细胞数量保持稳定。

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