接受¹³¹I治疗患者的适应性反应。

Adaptive response in patients treated with 131I.

作者信息

Monsieurs M A, Thierens H M, Vral A M, Van De Wiele C, De Ridder L I, Dierckx R A

机构信息

Department of Biomedical Physics and Radiation Protection, University of Gent, Belgium.

出版信息

J Nucl Med. 2000 Jan;41(1):17-22.

PMID:10647600

Abstract

UNLABELLED

The aim of this study was to investigate whether an adaptive response (defined as the induction of radiation tolerance after a small dose of radiation) could be observed in peripheral blood lymphocytes of patients treated with 1311 for thyroid disease.

METHODS

For each patient, blood samples were taken immediately before and 1 wk after 131I administration. Each blood sample was divided into 3 fractions and the fractions were subsequently irradiated in vitro with 0, 0.5, and 1.0 Gy 60Co gamma-rays. After blood culture for 70 h, cells were harvested and stained with Romanowsky-Giemsa and micronuclei were counted in 1000 binucleated cells. The increase in micronuclei by the in vitro irradiation of the blood samples taken before and after therapy was compared. In this setup, an adaptive response is represented by a significant decrease of the in vitro induced micronucleus yield after therapy compared with that before therapy. The iodine therapy can be considered as an in vivo adaptation dose, after which the subsequent in vitro irradiation acts as a challenge dose. To investigate the reproducibility of the method, 2 subsequent blood samples of healthy volunteers were taken 7 d apart. Irradiation and cell culture were performed as described.

RESULTS

In 8 of 20 patients, a significant (P = 0.0002) decrease was found in the in vitro induced micronucleus yield in the blood sample taken 1 wk after 1311 administration compared with that of the blood sample taken before therapy. No significant (P > 0.1) differences were observed between these 8 patients and the other patients when the number of micronuclei induced in vivo by the iodine treatment and the resulting equivalent total body dose were compared. None of the control subjects showed a significant change in micronucleus yield after in vitro irradiation between both blood samples taken 1 wk apart.

CONCLUSION

The iodine treatment can act as an in vivo adaptation dose and can induce an adaptive response that is observed by a decrease of the cytogenetic damage in peripheral blood lymphocytes after in vitro irradiation as a challenge dose. A large interindividual difference was observed.

摘要

未标记

本研究的目的是调查在接受¹³¹I治疗甲状腺疾病的患者外周血淋巴细胞中是否能观察到适应性反应（定义为小剂量辐射后诱导的辐射耐受性）。

方法

对于每位患者，在¹³¹I给药前和给药后1周立即采集血样。每个血样分为3份，随后这些份样本在体外分别用0、0.5和1.0 Gy的⁶⁰Coγ射线照射。血液培养70小时后，收获细胞并用罗曼诺夫斯基 - 吉姆萨染色，在1000个双核细胞中计数微核。比较治疗前后采集的血样经体外照射后微核的增加情况。在此设置中，适应性反应表现为治疗后体外诱导的微核产率与治疗前相比显著降低。碘治疗可被视为体内适应剂量，之后随后的体外照射作为激发剂量。为了研究该方法的可重复性，对健康志愿者相隔7天采集的2份后续血样进行检测。按照上述方法进行照射和细胞培养。

结果

20例患者中有8例，¹³¹I给药后1周采集的血样中体外诱导的微核产率与治疗前采集的血样相比显著降低（P = 0.0002）。当比较碘治疗在体内诱导的微核数量和由此产生的等效全身剂量时，这8例患者与其他患者之间未观察到显著差异（P>0.1）。在相隔1周采集的两份血样中，体外照射后，没有对照受试者的微核产率显示出显著变化。