Dinçer Zafer, Basan Hasan, Göger Nilgün Günden
Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, Etiler, Ankara 06330, Turkey.
J Pharm Biomed Anal. 2003 Apr 1;31(5):867-72. doi: 10.1016/s0731-7085(02)00664-7.
A derivative UV spectrophotometric method for the determination of ambroxol in tablets was developed. Determination of ambroxol in tablets was conducted by using first-order derivative UV spectrophotometric method at 255 nm (n = 5). Standards for the calibration graph ranging from 5.0 to 35.0 microg/ml were prepared from stock solution. The proposed method was accurate with 98.6+/-0.4% recovery value and precise with coefficient of variation (CV) of 1.22. These results were compared with those obtained by reference methods, zero-order UV spectrophotometric method and reversed-phase high-performance liquid chromatography (HPLC) method. A reversed-phase C(18) column with aqueous phosphate (0.01 M)-acetonitrile-glacial acetic acid (59:40:1, v/v/v) (pH 3.12) mobile phase was used and UV detector was set to 252 nm. Calibration solutions used in HPLC were ranging from 5.0 to 20.0 microg/ml. Results obtained by derivative UV spectrophotometric method was comparable to those obtained by reference methods, zero-order UV spectrophotometric method and HPLC, as far as ANOVA test, F(calculated) = 0.762 and F(theoretical) = 3.89, was concerned.
建立了一种用于测定片剂中氨溴索的导数紫外分光光度法。采用一阶导数紫外分光光度法在255 nm处（n = 5）对片剂中的氨溴索进行测定。由储备液制备校准曲线的标准品，浓度范围为5.0至35.0μg/ml。所提出的方法准确，回收率为98.6±0.4%，精密度良好，变异系数（CV）为1.22。将这些结果与参考方法（零阶紫外分光光度法和反相高效液相色谱法（HPLC））获得的结果进行比较。使用反相C(18)柱，流动相为磷酸盐缓冲液（0.01 M）-乙腈-冰醋酸（59:40:1，v/v/v）（pH 3.12），紫外检测器设定为252 nm。HPLC中使用的校准溶液浓度范围为5.0至20.0μg/ml。就方差分析测试而言，导数紫外分光光度法获得的结果与参考方法（零阶紫外分光光度法和HPLC）获得的结果具有可比性，F（计算值）= 0.762，F（理论值）= 3.89。
