浆液性积液中腺癌细胞以及恶性或反应性间皮细胞中的核糖核苷酸还原酶免疫反应性。

Ribonucleotide reductase immunoreactivity in adenocarcinoma cells and malignant or reactive mesothelial cells in serous effusions.

作者信息

Okamura Hiroshi, Kamei Toshiaki, Sakuma Nobuo, Hanai Nobuo, Ishihara Tokuhiro

机构信息

First Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan.

出版信息

Acta Cytol. 2003 Mar-Apr;47(2):209-15. doi: 10.1159/000326506.

DOI:10.1159/000326506

PMID:12685191

Abstract

OBJECTIVE

Ribonucleotide reductase (RNR) is a cytoplasmic enzyme that is essential for DNA synthesis. Its activity is strongly associated with cell proliferation. We assessed the value of immunostaining for RNR in distinguishing between reactive mesothelia (RM), malignant mesotheliomas (MM) and adenocarcinomas (AC) in serous effusions.

STUDY DESIGN

Cytocentrifuged cell smears of serous effusions from 38 RM, 10 MM and 36 AC were immunostained with the monoclonal antibody KM1054 raised against the R2 subunit of RNR (RNR-R2) using the labeled streptavidin-biotin method. Quantitative RNR-R2 values were determined by counting the percentages of immunoreactive cells.

RESULTS

RNR-R2 immunostaining was confined to the cytoplasm. The median RNR-R2 value was 1.4% (range, 0-7.9%) for RM, 11.2% (4.1-15.3%) for MM and 12.1% (2.0-40.6%) for AC. Significant differences in RNR-R2 values were found for both AC versus RM (P < .001) and MM versus RM (P = .009). There was no difference between AC and MM (P = .26). An RNR-R2 value > or = 7% was found in 30 of 36 AC, 8 of 10 MM and 2 of 38 RM.

CONCLUSION

RNR-R2 immunostaining can be useful as an adjunct for differentiating AC or MM from RM in serous effusions.

摘要

目的

核糖核苷酸还原酶（RNR）是一种对DNA合成至关重要的细胞质酶。其活性与细胞增殖密切相关。我们评估了RNR免疫染色在鉴别浆液性积液中的反应性间皮（RM）、恶性间皮瘤（MM）和腺癌（AC）中的价值。

研究设计

使用标记链霉亲和素-生物素法，用针对RNR的R2亚基（RNR-R2）产生的单克隆抗体KM1054对38例RM、10例MM和36例AC的浆液性积液细胞离心涂片进行免疫染色。通过计算免疫反应性细胞的百分比来确定RNR-R2的定量值。

结果

RNR-R2免疫染色局限于细胞质。RM的RNR-R2值中位数为1.4%（范围0-7.9%），MM为11.2%（4.1-15.3%），AC为12.1%（2.0-40.6%）。AC与RM（P<.001）以及MM与RM（P=.009）之间的RNR-R2值存在显著差异。AC和MM之间无差异（P=.26）。在36例AC中的30例、10例MM中的8例和38例RM中的2例中发现RNR-R2值≥7%。