脐血血浆中类固醇定量方法。

Method for the quantitation of steroids in umbilical cord plasma.

作者信息

Schindler A E, Sparke H

出版信息

Endocrinol Exp. 1975 Sep;9(3):205-14.

Abstract

A method for simultaneous quantitation of nine steroids in cord plasma is described which consists of Amberlite XAD-2-column chromatography at constant temperature of 45 degrees C, enzyme hydrolysis with beta-glucoronidase/aryl sulfatase, addition of five radioactive internal standards, ethyl acetate extraction, thin layer chromatography and quantitation by gas-liquid chromatography after trimethylsilyl ether derivative formation. Reliability criteria are established and the following steroid concentrations found: progesterone, 132.1 +/- 102.5 mug/100 ml; pregnenolone, 57.3 +/- 45.7 mug/100 ml; dehydroepiandrosterone, 46.5 +/- 29.4 mug/100 ml; pregnanediol, 67.5 +/- 46.6 mug/100 ml; 16-ketoandrostenediol 19.8 +/- 13.7 mug/100 ml; 16 alpha-hydroxydehydroepiandrosterone, 126.3 +/- 86.9 mug/100 ml; 16 alpha-hydroxypregnenolone, 78.2 +/- 56.5 mug/100 ml; androstenetriol, 22.2 +/- 17.5 mug/100 ml and oestriol, 127.7 +/- 116.9 mug/100.

摘要

本文描述了一种同时定量测定脐血血浆中九种甾体的方法，该方法包括在45℃恒温下进行Amberlite XAD - 2柱色谱法、用β - 葡萄糖醛酸酶/芳基硫酸酯酶进行酶水解、添加五种放射性内标、乙酸乙酯萃取、薄层色谱法以及在形成三甲基硅醚衍生物后通过气液色谱法定量。建立了可靠性标准，并得出以下甾体浓度：孕酮，132.1±102.5μg/100ml；孕烯醇酮，57.3±45.7μg/100ml；脱氢表雄酮，46.5±29.4μg/100ml；孕二醇，67.5±46.6μg/100ml；16 - 酮雄烯二醇19.8±13.7μg/100ml；16α - 羟基脱氢表雄酮，126.3±86.9μg/100ml；16α - 羟基孕烯醇酮，78.2±56.5μg/100ml；雄烯三醇，22.2±17.5μg/100ml；雌三醇，127.7±116.9μg/100。