O'Sullivan Michael, MacDougall Mary B, Unzeta Mercedes, Lizcano Jose-Miguel, Tipton Keith F
Department of Restorative Dentistry and Periodontology, Dublin Dental School and Hospital, Lincoln Place, Dublin 2, Ireland.
Biochim Biophys Acta. 2003 Apr 11;1647(1-2):333-6. doi: 10.1016/s1570-9639(03)00086-4.
The behaviour of semicarbazide-sensitive amine oxidase (SSAO; E.C. 1.4.3.6) in dental pulp has been studied, with particular reference to the metabolism of 5-hydroxytryptamine (5-HT; serotonin). Kinetic studies using radioactively labelled substrates have confirmed benzylamine, 2-phenylethylamine (PEA) and 5-HT to be substrates for microsomal SSAO from porcine dental pulp. Kinetic substrate-competition studies indicated the presence of two forms of SSAO in dental pulp; one that oxidises benzylamine and PEA but not 5-HT and a second that oxidises 5-HT and PEA but not benzylamine. These two forms also differ in their thermostabilities at 60 and 70 degrees C, although this thermal inactivation is partly reversible.
已对牙髓中氨基脲敏感胺氧化酶（SSAO；E.C. 1.4.3.6）的行为进行了研究，特别涉及5-羟色胺（5-HT；血清素）的代谢。使用放射性标记底物的动力学研究已证实苄胺、2-苯乙胺（PEA）和5-HT是猪牙髓微粒体SSAO的底物。动力学底物竞争研究表明牙髓中存在两种形式的SSAO；一种氧化苄胺和PEA但不氧化5-HT，另一种氧化5-HT和PEA但不氧化苄胺。这两种形式在60和70摄氏度下的热稳定性也有所不同，尽管这种热失活部分是可逆的。
