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2
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本文引用的文献

1
Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis.人类着丝粒蛋白I将着丝粒蛋白F、MAD1和MAD2定位到动粒上，对有丝分裂至关重要。
Nat Cell Biol. 2003 Apr;5(4):341-5. doi: 10.1038/ncb953.
2
BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1.BubR1对于其他纺锤体检查点蛋白在动粒上的定位至关重要，其磷酸化需要Mad1。
J Cell Biol. 2002 Aug 5;158(3):487-96. doi: 10.1083/jcb.200204048.
3
Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation.细胞质动力蛋白/动力蛋白激活蛋白驱动动粒蛋白向纺锤体极运输，并在有丝分裂纺锤体检查点失活中发挥作用。
J Cell Biol. 2001 Dec 24;155(7):1159-72. doi: 10.1083/jcb.200105093.
4
Kinetochore dynein: its dynamics and role in the transport of the Rough deal checkpoint protein.动粒动力蛋白：其动力学及在Rough deal检查点蛋白运输中的作用
Nat Cell Biol. 2001 Nov;3(11):1001-7. doi: 10.1038/ncb1101-1001.
5
The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore.ZW10和Rough Deal检查点蛋白在一个靶向动粒的大型、进化保守的复合物中共同发挥作用。
J Cell Sci. 2001 Sep;114(Pt 17):3103-14. doi: 10.1242/jcs.114.17.3103.
6
The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions.果蝇 CID 在动粒形成、细胞周期进程及异染色质相互作用中的作用。
Nat Cell Biol. 2001 Aug;3(8):730-9. doi: 10.1038/35087045.
7
The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants.在果蝇zw10和rod突变体中，染色体向极运动的速率会减弱。
Nat Cell Biol. 2000 Dec;2(12):948-52. doi: 10.1038/35046605.
8
Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores.人类Zw10和ROD是与动粒结合的有丝分裂检查点蛋白。
Nat Cell Biol. 2000 Dec;2(12):944-7. doi: 10.1038/35046598.
9
Rough deal and Zw10 are required for the metaphase checkpoint in Drosophila.在果蝇中，中期检查点需要Rough deal和Zw10。
Nat Cell Biol. 2000 Dec;2(12):939-43. doi: 10.1038/35046592.
10
CENP-E as an essential component of the mitotic checkpoint in vitro.着丝粒蛋白E作为体外有丝分裂检查点的重要组成部分。
Cell. 2000 Sep 15;102(6):817-26. doi: 10.1016/s0092-8674(00)00070-2.

Zwilch，一种着丝粒功能所需的ZW10/ROD复合物的新组分。

Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.

作者信息

Williams Byron C, Li ZeXiao, Liu Songtao, Williams Erika V, Leung Garmay, Yen Tim J, Goldberg Michael L

机构信息

Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853-2703, USA.

出版信息

Mol Biol Cell. 2003 Apr;14(4):1379-91. doi: 10.1091/mbc.e02-09-0624.

DOI:10.1091/mbc.e02-09-0624
PMID:12686595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC153108/
Abstract

The Zeste-White 10 (ZW10) and Rough Deal (ROD) proteins are part of a complex necessary for accurate chromosome segregation. This complex recruits cytoplasmic dynein to the kinetochore and participates in the spindle checkpoint. We used immunoaffinity chromatography and mass spectroscopy to identify the Drosophila proteins in this complex. We found that the complex contains an additional protein we name Zwilch. Zwilch localizes to kinetochores and kinetochore microtubules in a manner identical to ZW10 and ROD. We have also isolated a zwilch mutant, which exhibits the same mitotic phenotypes associated with zw10 and rod mutations: lagging chromosomes at anaphase and precocious sister chromatid separation upon activation of the spindle checkpoint. Zwilch's role within the context of this complex is evolutionarily conserved. The human Zwilch protein (hZwilch) coimmunoprecipitates with hZW10 and hROD from HeLa cell extracts and localizes to the kinetochores at prometaphase. Finally, we discuss immunoaffinity chromatography results that suggest the existence of a weak interaction between the ZW10/ROD/Zwilch complex and the kinesin-like kinetochore component CENP-meta.

摘要

Zeste-White 10（ZW10）蛋白和Rough Deal（ROD）蛋白是精确染色体分离所必需的一个复合体的组成部分。该复合体将细胞质动力蛋白招募到动粒，并参与纺锤体检查点。我们利用免疫亲和层析和质谱技术来鉴定该复合体中的果蝇蛋白。我们发现该复合体包含一种我们命名为Zwilch的额外蛋白。Zwilch以与ZW10和ROD相同的方式定位于动粒和动粒微管。我们还分离出了一个zwilch突变体，它表现出与zw10和rod突变相关的相同有丝分裂表型：后期染色体滞后以及纺锤体检查点激活时姐妹染色单体过早分离。Zwilch在这个复合体中的作用在进化上是保守的。人Zwilch蛋白（hZwilch）可从HeLa细胞提取物中与hZW10和hROD进行共免疫沉淀，并在前中期定位于动粒。最后，我们讨论了免疫亲和层析结果，这些结果表明ZW10/ROD/Zwilch复合体与类驱动蛋白动粒成分CENP-meta之间存在弱相互作用。