Liu Runjin, Wang Fayuan
Mycorrhiza Laboratory, Laiyang Agricultural College, Laiyang, Shandong Province 265200, PR China.
Mycorrhiza. 2003 Jun;13(3):123-7. doi: 10.1007/s00572-002-0207-4. Epub 2003 Mar 29.
Arbuscular mycorrhizal (AM) fungi in coalmine spoil, island forest and saline soils were enriched in pot culture with maize (Zea mays L.), tobacco (Nicotiana tabacum L.), white clover (Trifolium repens Linn.) and silverweed cinquefoil (Potentilla anserina L.). Based on spores, there were more species of AM fungi in the coalmine spoil (15 species, 3 genera), than in the forest soil (11 species, 4 genera) and the saline soil (5 species, 2 genera). In the trap cultures, the total of 28 species in Acaulospora, Gigaspora, Glomus, and Sclerocystis detected in the original soils were all recovered with at least one of the four trap plants. The highest spore and species numbers were recovered in trap cultures of T. repens inoculated with coalmine spoil. Glomus constrictum and Glomus multicaule were the dominant species associated with N. tabacum grown in saline soil and forest soil. The dominant species of AM fungi on the four hosts was Acaulospora mellea, which had over 90% of the spore incidence in pot trap culture in coalmine spoil. It is suggested that there be selectivity between host plants and AM fungi. The number of species of AM fungi detected was influenced by host plants under certain conditions and white clover was generally the optimal host plant to detect diversity of AM fungi.
通过盆栽玉米（Zea mays L.）、烟草（Nicotiana tabacum L.）、白三叶草（Trifolium repens Linn.）和鹅绒委陵菜（Potentilla anserina L.），对煤矿废弃地、海岛森林和盐碱地中的丛枝菌根（AM）真菌进行了富集培养。基于孢子数量，煤矿废弃地中的AM真菌种类（15种，3个属）比森林土壤（11种，4个属）和盐碱地（5种，2个属）更多。在诱捕培养中，在原始土壤中检测到的无梗囊霉属、巨孢囊霉属、球囊霉属和硬囊霉属中的28种AM真菌，均能在四种诱捕植物中的至少一种上回收得到。接种煤矿废弃地土壤的白三叶草诱捕培养中回收得到的孢子和种类数量最多。缩球囊霉（Glomus constrictum）和多梗球囊霉（Glomus multicaule）是与生长在盐碱地和森林土壤中的烟草相关的优势种。在四种宿主植物上，AM真菌的优势种是蜜色无梗囊霉（Acaulospora mellea），在煤矿废弃地盆栽诱捕培养中其孢子发生率超过90%。这表明宿主植物与AM真菌之间存在选择性。在一定条件下，宿主植物会影响检测到的AM真菌种类数量，白三叶草通常是检测AM真菌多样性的最佳宿主植物。
