Tenzin Ugyen Wangmo, Noirungsee Nuttapol, Runsaeng Phanthipha, Noppradit Prakrit, Klinnawee Lompong
Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
Plant Cell and Physiology for Sustainable Agriculture Research Unit, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
J Fungi (Basel). 2022 Oct 10;8(10):1061. doi: 10.3390/jof8101061.
The use of arbuscular mycorrhizal fungi (AMF) as biofertilizer in agriculture is a sustainable approach to fertilization. The first step in the production of AMF biofertilizer is inoculation of mycotrophic plants with a composite of soil and native plant roots, containing potentially viable AMF spores from natural habitats, to a trap culture. A single host plant or a consortium of host plants can be used to propagate AMF spores. However, the difference in the comparative efficiency of mono- and co-cultivated host plants used for the production of AMF spores and the maintenance of original AMF community composition has not been well elucidated. Here, we prepared trap culture with nutrient-poor soil from coastal sand dune vegetation collected during the dry season when the AMF spore density and relative abundance of Glomeromycota ITS2 sequences were significantly higher (p = <0.05) than in the wet season. The AMF communities in the soil were mainly composed of Glomus spp. Maize (Zea mays L.) and/or Sorghum (Sorghum bicolor (L.). Moench) were grown in trap cultures in the greenhouse. Our results demonstrated that co-cultivation of the host plants increased the production of AMF spores but, compared to mono-cultivation of host plants, did not better sustain the native AMF community compositions in the coastal sand dune soil. We propose that the co-cultivation of host plants in a trap culture broadens AMF-host plant compatibilities and thus sustains the symbiotic association of the natively diverse AMF. Therefore, the results of this study suggest that further research is needed to confirm whether the co-culturing of more than one host plant is as efficient a strategy as using a monoculture of a single host plant.
在农业中使用丛枝菌根真菌（AMF）作为生物肥料是一种可持续的施肥方法。生产AMF生物肥料的第一步是将含有来自自然栖息地潜在存活AMF孢子的土壤和本地植物根系复合物接种到诱捕培养物中的菌根营养植物上。可以使用单一宿主植物或宿主植物联合体来繁殖AMF孢子。然而，用于生产AMF孢子的单作和混作宿主植物的比较效率差异以及原始AMF群落组成的维持尚未得到充分阐明。在这里，我们用旱季采集的沿海沙丘植被的贫营养土壤制备了诱捕培养物，此时AMF孢子密度和球囊菌门ITS2序列的相对丰度显著高于雨季（p = <0.05）。土壤中的AMF群落主要由球囊霉属物种组成。玉米（Zea mays L.）和/或高粱（Sorghum bicolor (L.). Moench）在温室中的诱捕培养物中生长。我们的结果表明，宿主植物的混作增加了AMF孢子的产量，但与宿主植物的单作相比，并没有更好地维持沿海沙丘土壤中本地AMF群落的组成。我们提出，在诱捕培养物中宿主植物的混作拓宽了AMF与宿主植物的兼容性，从而维持了本地多样AMF的共生关系。因此，本研究结果表明，需要进一步研究以确认多种宿主植物的混作是否与使用单一宿主植物的单作一样有效。
