使用共固定化酶反应器的甘油和甘油三酯流动注射化学发光分析法

Flow injection chemiluminescent assays for glycerol and triglycerides using a co-immobilized enzyme reactor.

作者信息

Yaqoob M, Nabi A

机构信息

Department of Chemistry, University of Balochistan, Quetta, Pakistan.

出版信息

Luminescence. 2003 Mar-Apr;18(2):67-71. doi: 10.1002/bio.704.

DOI:10.1002/bio.704

PMID:12687625

Abstract

A flow injection method for the determination of glycerol using a co-immobilized enzyme reactor containing glycerokinase and glycerol-3-phosphate oxidase is described. The hydrogen peroxide produced is monitored by using a luminol chemiluminescence reaction in the presence of catalyst such as Co(II). The detection limit (2.5 x blank noise) for glycerol is 7 x 10(-3) mmol/L with a sample throughput of 40/h. The calibration graph is linear over the range studied (0.2-1.0 mmol/L) with relative standard deviation 1.2-2.4%. The method is applied to the determination of glycerol in blood serum produced off-line from triglycerides using lipase isolated from bovine pancreas.

摘要

描述了一种流动注射法，用于使用包含甘油激酶和甘油-3-磷酸氧化酶的共固定化酶反应器测定甘油。产生的过氧化氢通过在诸如Co(II)等催化剂存在下的鲁米诺化学发光反应进行监测。甘油的检测限（2.5倍空白噪声）为7×10(-3) mmol/L，样品通量为40个/小时。在所研究的范围内（0.2 - 1.0 mmol/L）校准曲线呈线性，相对标准偏差为1.2 - 2.4%。该方法应用于使用从牛胰腺分离的脂肪酶离线从甘油三酯产生的血清中甘油的测定。