Kalia Vandana, Pundir Chandra S
Biochemistry Research Laboratory, Department of BioSciences, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
Indian J Biochem Biophys. 2004 Dec;41(6):326-8.
A method for determination of serum triglycerides (Tgs) using lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase co-immobilized onto alkylamine glass beads (pore diameter 55 nm) through glutaraldehyde coupling was developed and evaluated. The minimum detection limit of the method was 0.54 mM. The analytical recovery of added triolein in the serum was 97.55 +/- 1.5% (mean +/- S.D.). The mean value of serum Tgs, determined by the present method showed a good correlation (r = 0.984) with the Bayer's kit method, employing free enzymes. The within and between batch coefficients of variation (CV) were < 2.25% and < 1.35% respectively. No significant loss of activity was observed, when co-immobilized enzymes were reused for about 200 times and stored at 4 degrees C in distilled water. The cost of Tg determination for 200 serum samples was less, as compared with Bayer's kit method.
开发并评估了一种通过戊二醛偶联将脂肪酶、甘油激酶、甘油-3-磷酸氧化酶和过氧化物酶共固定在烷基胺玻璃珠（孔径55nm）上以测定血清甘油三酯（Tg）的方法。该方法的最低检测限为0.54mM。血清中添加的三油精的分析回收率为97.55±1.5%（平均值±标准差）。用本方法测定的血清Tg平均值与采用游离酶的拜耳试剂盒方法具有良好的相关性（r=0.984）。批内和批间变异系数（CV）分别<2.25%和<1.35%。当共固定化酶重复使用约200次并在4℃下于蒸馏水中储存时，未观察到明显的活性损失。与拜耳试剂盒方法相比，测定200份血清样本的Tg成本更低。
