肽基转移酶抑制剂对核糖核酸酶P活性的抑制动力学。抗生素对核糖核酸酶P的影响。

Kinetics of inhibition of ribonuclease P activity by peptidyltransferase inhibitors. Effect of antibiotics on RNase P.

作者信息

Kalavrizioti Dimitra, Vourekas Anastassios, Tekos Apostolos, Tsagla Antigoni, Stathopoulos Constantinos, Drainas Denis

机构信息

Department of Biochemistry, School of Medicine, University of Patras, 26500 Patras, Greece.

出版信息

Mol Biol Rep. 2003 Mar;30(1):9-14. doi: 10.1023/a:1022290110116.

DOI:10.1023/a:1022290110116

PMID:12688530

Abstract

A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S. Detailed kinetic analysis showed that the type of inhibition of RNase P activity by puromycin is simple competitive, whereas the type of inhibition by amicetin and blasticidin S is simple non-competitive. On the basis of Ki values amicetin is stronger inhibitor than puromycin and blasticidin S.

摘要

一种源自盘基网柄菌的无细胞系统已被用于研究嘌呤霉素、杀稻瘟菌素和杀稻瘟素S对核糖核酸酶P的抑制动力学。详细的动力学分析表明，嘌呤霉素对核糖核酸酶P活性的抑制类型为简单竞争性抑制，而杀稻瘟菌素和杀稻瘟素S的抑制类型为简单非竞争性抑制。基于抑制常数（Ki）值，杀稻瘟菌素是比嘌呤霉素和杀稻瘟素S更强的抑制剂。