Xu Jian, Ji Chaoneng, Wang Liu, Cao Yueqiong, Dai Jianliang, Ye Xin, Zeng Li, Dai Jianfeng, Wu Qihan, Xie Yi, Mao Yumin
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Mol Biol Rep. 2003 Mar;30(1):47-52. doi: 10.1023/a:1022250428015.
We report here cloning and characterization of a novel human gene, termed C5orf12, which is a putative membrane protein belonging to the TMS_TDE family. The cDNA encodes 423 amino acid with a putative molecular weight of about 47 KDa. Secondary structure prediction showed that C5orf12 contained 10 putative transmembrane helices, which has high identity with other family members. We performed RT-PCR to examine its expression pattern. The result showed that C5orf12 was highly expressed in placenta, skeletal muscle, spleen, thymus, testis and peripheral leukocyte while expressed weakly in heart and liver. C5orf12 has high identity with the rat TPO1, so we speculate that C5orf12 may also have a role in the brain development.
我们在此报告一个名为C5orf12的新型人类基因的克隆与特性分析，它是一种属于TMS_TDE家族的推定膜蛋白。该cDNA编码423个氨基酸，推定分子量约为47 kDa。二级结构预测表明，C5orf12含有10个推定的跨膜螺旋，与其他家族成员具有高度同源性。我们进行了RT-PCR以检测其表达模式。结果显示，C5orf12在胎盘、骨骼肌、脾脏、胸腺、睾丸和外周血白细胞中高表达，而在心脏和肝脏中表达较弱。C5orf12与大鼠TPO1具有高度同源性，因此我们推测C5orf12可能在大脑发育中也发挥作用。
