胸苷及其类似物对抗体形成细胞分化的影响。

The effects of thymidine and its analogues on the differentiation of antibody-forming cells.

作者信息

Harris G, Walford E M, Olsen I

出版信息

Differentiation. 1975 Aug 11;3(1-3):99-105. doi: 10.1111/j.1432-0436.1975.tb00849.x.

DOI:10.1111/j.1432-0436.1975.tb00849.x

Abstract

Thymidine (Tdr) and its analogues 5-fluorodeoxyuridine (FUdr) and 5-bromodeoxyuridine (BUdr) inhibited the development of antibody-producing cells (PFC) in cultures of explants of rabbit spleen stimulated with sheep red cells in vitro. They were effective in this respect, even at concentrations too low to inhibit cell division. The results indicated that Tdr had produced its effects by a selective action on DNA and RNA synthesis. In view of what is known about the mechanisms of action of Tdr and its analogues to interfere with DNA-dependent events in cells, these results supported the idea that DNA synthesis, unrelated to the needs for cell division, was required for the development of antibody-producing capacity by PFC in these cultures.

摘要

胸腺嘧啶核苷（Tdr）及其类似物5-氟脱氧尿苷（FUdr）和5-溴脱氧尿苷（BUdr）在体外抑制用绵羊红细胞刺激的兔脾外植体培养物中抗体产生细胞（PFC）的发育。即使在浓度低至不足以抑制细胞分裂的情况下，它们在这方面也是有效的。结果表明，Tdr通过对DNA和RNA合成的选择性作用产生了其效应。鉴于已知Tdr及其类似物干扰细胞中依赖DNA事件的作用机制，这些结果支持了这样一种观点，即在这些培养物中，PFC产生抗体能力的发育需要与细胞分裂需求无关的DNA合成。