Harris G
J Exp Med. 1968 Apr 1;127(4):675-91. doi: 10.1084/jem.127.4.675.
The effects of actinomycin D and puromycin on spleen cell suspensions from rabbits immunized to SRC's were studied. These inhibitors, in high concentration, suppressed PFC's when added initially to recently isolated cells. When such cells were incubated for several days in the presence of both antigen and inhibitor, both actinomycin D and puromycin produced an increase in PFC's after the initial suppression. This recovery effect was best seen with cells from rabbits killed 3 days after boosting with SRC's, and was usually absent when cells were taken from rabbits killed 2 days after boosting. When actinomycin D or puromycin was added after several days in culture in the presence of SRC's, surviving PFC's were found to be not only resistant to these inhibitors, but there was also an increased number of PFC's compared to similar cultures incubated without these agents. Radioautographic studies showed that PFC's stimulated by the presence of actinomycin D or puromycin were not incorporating precursors for RNA or protein synthesis. In view of the known mode of action of these inhibitors, it was postulated that they were stimulating antibody production by PFC's in vitro either by interfering with represser mechanisms or stimulating the completion of antibody molecules, perhaps by causing the release of preformed antibody chains from ribosomes. Since the presence of specific antigens in vitro were necessary for these observed stimulatory effects on PFC's, and since antigens were producing an effect on antibody production on cells which were being suppressed by these inhibitors, added initially, it was further suggested that one role of antigen in the immune response was concerned with the completion of antibody synthesis on the ribosomes, perhaps by acting as an inducer as has been suggested previously (1).
研究了放线菌素D和嘌呤霉素对用肉瘤细胞（SRC）免疫的家兔脾细胞悬液的影响。当最初将这些抑制剂以高浓度添加到刚分离的细胞中时，它们会抑制空斑形成细胞（PFC）。当这些细胞在抗原和抑制剂存在的情况下孵育数天时，放线菌素D和嘌呤霉素在最初的抑制作用后都会使PFC数量增加。这种恢复效应在用SRC加强免疫后3天处死的家兔的细胞中最为明显，而在用SRC加强免疫后2天处死的家兔中取的细胞通常没有这种效应。当在存在SRC的情况下培养数天后添加放线菌素D或嘌呤霉素时，发现存活的PFC不仅对这些抑制剂有抗性，而且与未添加这些试剂的类似培养物相比，PFC的数量也增加了。放射自显影研究表明，由放线菌素D或嘌呤霉素刺激产生的PFC没有掺入RNA或蛋白质合成的前体。鉴于这些抑制剂已知的作用方式，推测它们在体外通过干扰阻遏机制或刺激抗体分子的完成来刺激PFC产生抗体，也许是通过使预先形成的抗体链从核糖体释放出来。由于体外存在特异性抗原对于观察到的对PFC的这些刺激作用是必要的，并且由于抗原对最初添加的这些抑制剂所抑制的细胞的抗体产生有影响，因此进一步表明抗原在免疫反应中的一个作用与核糖体上抗体合成的完成有关，也许就像先前有人提出的那样（1），抗原起到诱导剂的作用。
