Myers D K, Feinendegen L E
Cell Tissue Kinet. 1976 May;9(3):215-21. doi: 10.1111/j.1365-2184.1976.tb01269.x.
Mice were injected intravenously with tritiated thymidine (TdR) and 125I-labeled iododeoxyuridine (IUdR) in low doses to provide a simultaneous labeling of tissue DNA with non-toxic amounts of these two precursors. The total activity per organ and the ratio of the two isotopes was measured in the DNA at various times between 1 and 15 days after the injection. Since TdR from dying cells is re-utilized more effeciently than IUdR from the same cells, more labeled TdR than IUdR was retained in the tissue DNA in these experiments. From the slopes of the regression lines, the true rated of turnover of replicating tissue DNA and the per cent re-utilization of TdR were calculated. Re-utilization of TdR varied from 37 to 60% in the six tissues examined.
给小鼠静脉注射低剂量的氚标记胸腺嘧啶核苷（TdR）和¹²⁵I标记碘脱氧尿苷（IUdR），以便用无毒量的这两种前体同时标记组织DNA。在注射后1至15天的不同时间测量每个器官的总活性以及DNA中两种同位素的比例。由于来自死亡细胞的TdR比来自相同细胞的IUdR更有效地被重新利用，在这些实验中，组织DNA中保留的标记TdR比IUdR更多。根据回归线的斜率，计算复制组织DNA的真正周转速率和TdR的再利用率。在所检查的六种组织中，TdR的再利用率在37%至60%之间变化。
