Chatterjee P
Cell Tissue Res. 1976 Apr 2;167(3):387-405. doi: 10.1007/BF00219150.
Pars intermedia (PI) tissue from fetal, perinatal, neonatal and juvenile rabbits has been maintained in organ culture for up to nine weeks after explantation. Autoradiography showed that DNA synthesis took place for at least 22 days of culturing. PI-glandular cells and interstitial cells remain identifiable throughout this period but ACT-type cells were recognised only up to six weeks. Material from fetal and perinatal animals had a higher proportion of surviving cells than that from adult animals. The degree of differentiation achieved by PI-glandular cells in vitro appears to depend on three factors: i) the stage of development reached before explantation; ii) the original topographic position in the PI tissue before explantation; and iii) the position in the explant in relation to the gas-liquid interphase.
取自胎儿、围产期、新生儿和幼年兔子的中间部（PI）组织在移出后已在器官培养中维持了长达九周。放射自显影显示，在培养的至少22天内发生了DNA合成。在整个这段时间内，PI腺细胞和间质细胞仍然可以识别，但仅在长达六周时能识别出促肾上腺皮质激素（ACT）型细胞。来自胎儿和围产期动物的材料比来自成年动物的材料具有更高比例的存活细胞。PI腺细胞在体外达到的分化程度似乎取决于三个因素：i）移出前达到的发育阶段；ii）移出前在PI组织中的原始地形位置；iii）移出物中相对于气液界面的位置。
