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培养对兔腺垂体解离细胞超微结构的影响。

The effect of culture on ultrastructure of dissociated rabbit adenohypophysial cells.

作者信息

Chatterjee P

出版信息

J Anat. 1976 Apr;121(Pt 2):241-58.

PMID:179968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1231797/
Abstract

Cells from the rabbit pars distalis and pars intermedia have been cultured in several ways. In clump cultures the glandular adenohypophysial cells of the primary clump remain identifiable for long periods. Both acidophil and mucoid cells can be maintained in this way for over 50 days. During this time two different modes of granule extrusion are observed, one being found only in acidophil cells and the other being exclusive to mucoid cells. From this it is concluded that specific release of secretory products from these cells can occur in the absence of hypothalmic control. A special form of collagenous fibre is produced in clump cultures. Monolayer cell cultures are derived either from isolated adenohypophysial cells or from secondarily aggregated clumps, but in either case the predominant cells are fibroblasts, which persist for over 50 days (as isolated cultures) and for over 90 days (as spreads from clumps). Rounded cells can be recognized as a separate type in isolated cell culture.

摘要

兔远侧部和中间部的细胞已通过多种方法进行培养。在团块培养中，原代团块中的腺垂体腺细胞可长时间保持可识别状态。嗜酸性细胞和黏液样细胞都能以这种方式维持50多天。在此期间，观察到两种不同的颗粒排出模式，一种仅见于嗜酸性细胞，另一种则为黏液样细胞所特有。由此得出结论，在没有下丘脑控制的情况下，这些细胞也能发生分泌产物的特异性释放。团块培养中会产生一种特殊形式的胶原纤维。单层细胞培养物要么来自分离的腺垂体细胞，要么来自二次聚集的团块，但在任何一种情况下，主要细胞都是成纤维细胞，它们能持续存在50多天（作为分离培养物）和90多天（作为从团块铺展而来的细胞）。在分离细胞培养中，圆形细胞可被识别为一种单独的类型。

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IN VITRO REINITIATION OF PITUITARY SOMATOTROPIN RELEASE BY AN ACID EXTRACT OF HYPOTHALAMUS.下丘脑酸性提取物对垂体生长激素释放的体外再启动
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