Noguchi Eitaro, Ohsawa Hideo, Tanaka Hideki, Ikeda Hiroko, Aikawa Yoshihiro
Laboratory of Acupuncture, Tsukuba College of Technology, Tsukuba, 305-0821 Japan.
Jpn J Physiol. 2003 Feb;53(1):1-7. doi: 10.2170/jjphysiol.53.1.
The effect of electro-acupuncture stimulation (EAS) on duodenal motility was examined in anesthetized, artificially ventilated rats. EAS was applied to the abdominal area or to a hindpaw for 30 s at stimulus intensities of 0.1-10.0 mA with a stimulus frequency of 20 Hz. The duodenal motility was measured using the balloon method at a position about 1.5 cm caudal from the pylorus. Duodenal motility was inhibited by EAS at intensities of more than 5.0 mA (suprathreshold of group IV afferent excitation) when applied to the abdominal area. The duodenal inhibitory response existed after bilateral vagotomy or spinal transection, but was abolished by sectioning bilateral splanchnic nerves. Duodenal motility was facilitated by EAS at intensities of more than 2.0 mA (subthreshold of group IV, and suprathreshold for groups II+III afferent excitation) when applied to a hindpaw. The duodenal facilitatory response by EAS to a hindpaw existed after sectioning the splanchnic nerves, but disappeared after bilateral vagotomy or spinal transection. Furthermore, repetitive electrical stimulation of vagal efferent nerves enhanced duodenal motility, while repetitive electrical stimulation of the splanchnic efferent nerves inhibited the motility. It was concluded that the inhibitory response of duodenal motility elicited by EAS to the abdominal area is a spinal reflex response involving splanchnic inhibitory efferent nerves, and the enhanced response of duodenal motility by EAS to a hindpaw is a supraspinal reflex response involving vagal excitatory nerves.
在麻醉、人工通气的大鼠中研究了电针刺激（EAS）对十二指肠运动的影响。将EAS施加于腹部区域或后爪，刺激强度为0.1 - 10.0 mA，刺激频率为20 Hz，持续30秒。使用气囊法在距幽门约1.5 cm尾侧的位置测量十二指肠运动。当EAS以大于5.0 mA的强度（IV组传入神经兴奋阈值以上）施加于腹部区域时，十二指肠运动受到抑制。双侧迷走神经切断术或脊髓横断术后，十二指肠抑制反应仍然存在，但双侧内脏神经切断后该反应消失。当EAS以大于2.0 mA的强度（IV组阈值以下，II + III组传入神经兴奋阈值以上）施加于后爪时，十二指肠运动得到促进。EAS对后爪的十二指肠促进反应在内脏神经切断后仍然存在，但在双侧迷走神经切断术或脊髓横断术后消失。此外，反复电刺激迷走神经传出神经可增强十二指肠运动，而反复电刺激内脏神经传出神经则抑制该运动。得出的结论是，EAS对腹部区域引起的十二指肠运动抑制反应是一种涉及内脏抑制性传出神经的脊髓反射反应，而EAS对后爪引起的十二指肠运动增强反应是一种涉及迷走神经兴奋性神经的脊髓上反射反应。
