Abrudan O, Dejica D
Clin Chim Acta. 1976 May 17;69(1):113-5. doi: 10.1016/0009-8981(76)90479-4.
A method is described for increasing the sensitivity of conventional counterimmunoelectrophoresis (CIEP), i.e. double sequential counterimmunoelectrophoresis (DCIEP). Our method consists of a second electrophoretic migration made after the classical CIEP, where instead of HB antibody (from polytransfused patients), the anodic wells are filled with an antihuman-gammaglobulin antiserum. DCIEP is significantly more sensitive than CIEP (P less than 0.01), the number of positive sera in chronic lever diseases being 28.7% compared with 17.5%.
本文描述了一种提高传统对流免疫电泳（CIEP）灵敏度的方法，即双重连续对流免疫电泳（DCIEP）。我们的方法包括在经典CIEP之后进行第二次电泳迁移，此时阳极孔中填充的不是（来自多次输血患者的）乙肝抗体，而是抗人γ球蛋白抗血清。DCIEP的灵敏度显著高于CIEP（P<0.01），慢性肝病阳性血清的比例为28.7%，而CIEP为17.5%。
