• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Construction and characterization of pentacistronic retrovirus vectors.

作者信息

Felipe Pablo de, Izquierdo Marta

机构信息

Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain.

出版信息

J Gen Virol. 2003 May;84(Pt 5):1281-1285. doi: 10.1099/vir.0.18998-0.

DOI:10.1099/vir.0.18998-0
PMID:12692295
Abstract

The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size approximately 7.9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.

摘要

相似文献

1
Construction and characterization of pentacistronic retrovirus vectors.
J Gen Virol. 2003 May;84(Pt 5):1281-1285. doi: 10.1099/vir.0.18998-0.
2
Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons.含有口蹄疫病毒2A序列的重组腺相关病毒载体在培养细胞和大鼠黑质神经元中实现高效双顺反子基因表达。
Gene Ther. 2001 Jun;8(11):864-73. doi: 10.1038/sj.gt.3301469.
3
Tricistronic and tetracistronic retroviral vectors for gene transfer.用于基因转移的三顺反子和四顺反子逆转录病毒载体。
Hum Gene Ther. 2000 Sep 1;11(13):1921-31. doi: 10.1089/10430340050129530.
4
Translation driven by picornavirus IRES is hampered from Sindbis virus replicons: rescue by poliovirus 2A protease.由小核糖核酸病毒 IRES 驱动的翻译受到辛德比斯病毒复制子的阻碍：由脊髓灰质炎病毒 2A 蛋白酶挽救。
J Mol Biol. 2010 Sep 10;402(1):101-17. doi: 10.1016/j.jmb.2010.07.014. Epub 2010 Jul 17.
5
Functional expression of secreted proteins from a bicistronic retroviral cassette based on foot-and-mouth disease virus 2A can be position dependent.基于口蹄疫病毒2A的双顺反子逆转录病毒盒中分泌蛋白的功能表达可能取决于位置。
Hum Gene Ther. 2010 Nov;21(11):1631-7. doi: 10.1089/hum.2009.197.
6
A uniquely stable replication-competent retrovirus vector achieves efficient gene delivery in vitro and in solid tumors.一种具有独特稳定性且具备复制能力的逆转录病毒载体在体外和实体瘤中均能实现高效的基因递送。
Hum Gene Ther. 2001 May 20;12(8):921-32. doi: 10.1089/104303401750195881.
7
Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI.包含口蹄疫病毒2A切割因子的多顺反子慢病毒载体在有限感染复数下可实现编码基因的强劲表达。
Virol J. 2006 Mar 15;3:14. doi: 10.1186/1743-422X-3-14.
8
Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications.优化口蹄疫病毒 2A 共表达系统用于生物医学应用。
BMC Biotechnol. 2013 Aug 22;13:67. doi: 10.1186/1472-6750-13-67.
9
High-titer bicistronic retroviral vectors employing foot-and-mouth disease virus internal ribosome entry site.采用口蹄疫病毒内部核糖体进入位点的高滴度双顺反子逆转录病毒载体。
Nucleic Acids Res. 1996 Jul 15;24(14):2697-700. doi: 10.1093/nar/24.14.2697.
10
Type and position of promoter elements in retroviral vectors have substantial effects on the expression level of an enhanced green fluorescent protein reporter gene.逆转录病毒载体中启动子元件的类型和位置对增强型绿色荧光蛋白报告基因的表达水平有显著影响。
J Cancer Res Clin Oncol. 2000 Jul;126(7):391-9. doi: 10.1007/pl00008487.

引用本文的文献

1
Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Showing High Antimicrobial Activity.抗脂多糖因子异构体 3 的表达在显示高抗菌活性中。
Mar Drugs. 2021 Apr 23;19(5):239. doi: 10.3390/md19050239.
2
Viral nanoparticles as macromolecular devices for new therapeutic and pharmaceutical approaches.作为新型治疗和制药方法的大分子装置的病毒纳米颗粒。
Int J Physiol Pathophysiol Pharmacol. 2010 Jul 6;2(2):161-178.
3
Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors.三顺反子逆转录病毒载体中不同内部核糖体进入位点（IRES）的应用与比较
BMC Biotechnol. 2004 Jul 27;4:16. doi: 10.1186/1472-6750-4-16.