资源猪群体中肌肉蛋白质含量数量性状位点的检测

Detection of quantitative trait loci for protein percentage in muscle in resource pig population.

作者信息

Jiang Xun-Ping, Xiong Yuan-Zhu, Deng Chang-Yan, Qu Yan-Chun, Liu Gui-Qiong

机构信息

Key Laboratory of Swine Breeding and Genetics Under Agricultural Ministry, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Yi Chuan Xue Bao. 2002 Dec;29(12):1068-72.

PMID:12693097

Abstract

An intercross between Meishan and Large White pig population was used to map quantitative trait loci(QTL) for protein percentage in muscle. In the present study, all 135 F2 animals, their parents and their grandparents were genotyped for 24 microsatellites, which were in 1, 2 and 6 chromosome. The marker genotypes were used to calculate the additive and dominant coefficients at fixed position in the genome of each F2 animal, and the protein percentage were regressed on to the coefficients in intervals of 1 cM. Three significant QTLs effects were found for protein percentage in muscle. They were located between markers Sw1332 and Sw2130 at chromosome 1, between Sw2516 and Sw1201 at chromosome 2 and between Sw322 and Sw607 at chromosome 6(P < 0.05), and the explained residual variance were 0.91%, 12.76% and 4.60%, respectively. The results show that QTLs, which affect protein percentage, may be located in different chromosomes.

摘要

利用梅山猪和大白猪群体的杂交来定位肌肉中蛋白质含量的数量性状基因座（QTL）。在本研究中，对135只F2代动物及其亲本和祖代进行了位于1号、2号和6号染色体上的24个微卫星标记的基因分型。利用标记基因型计算每只F2代动物基因组中固定位置的加性和显性系数，并以1厘摩的间隔将蛋白质含量对这些系数进行回归分析。发现了3个对肌肉蛋白质含量有显著影响的QTL。它们分别位于1号染色体上的标记Sw1332和Sw2130之间、2号染色体上的Sw2516和Sw1201之间以及6号染色体上的Sw322和Sw607之间（P < 0.05），所解释的剩余方差分别为0.91%、12.76%和4.60%。结果表明，影响蛋白质含量的QTL可能位于不同的染色体上。