Qu Yan-Chun, Deng Chang-Yan, Xiong Yuan-Zhu, Zheng Rong, Yu Li, Su Yu-Hong, Liu Gui-Lan
Key Laboratory of Pig Breeding and Genetics, Agricultural Ministry, Huazhong Agricultural University, Wuhan 430070, China.
Yi Chuan Xue Bao. 2002;29(11):972-6.
The study constructed the genetic linkage map of porcine chromosome 2 and further analysis of quantitative trait loci was conducted. The results of the study demonstrated that all 7 microsatellite loci we chose were with relatively high polymorphism, and its polymorphic information content was from 0.40182 to 0.58477. The genetic map we constructed for resource family was 152.9 cM in length, with the order of all loci highly consistent with the USDA map. All marker intervals were longer than USDA map with the interval between marker Sw2516 and Sw1201 as an exception. Furthermore, we conducted QTLs locating analysis by combining the genetic map with the phenotypic data. QTLs affecting lively estimated traits such as lean meat percentage, were located at 60-65 cM on chromosome 2, while QTLs for the height and marbling of Longissmus dorsi muscle were located at 20 cM and 55 cM, respectively Among them, QTL for estimated lean meat percentage was significant at chromosome-wise level (P < 0.01) and was responsible for 21.55% of the phenotypic variance. QTLs for the height and marbling of Longissmus dorsi muscle were responsible for 10.12% and 10.97% of the phenotypic variance, respectively. The additive and dominance effect of lively estimated traits were in the inverse tendency, while the QTL for the height of Longissmus dorsi muscle had its additive and dominance effect in the same tendency and was with advantageous allele in Large White. The QTLs we detected had relatively large effect on phenotype and built a basis for molecular marker assisted selection and breeding.
本研究构建了猪2号染色体的遗传连锁图谱，并进一步进行了数量性状位点分析。研究结果表明，我们选择的7个微卫星位点均具有较高的多态性，其多态信息含量在0.40182至0.58477之间。我们为资源家系构建的遗传图谱长度为152.9 cM，所有位点的顺序与美国农业部图谱高度一致。除标记Sw2516和Sw1201之间的间隔外，所有标记区间均长于美国农业部图谱。此外，我们将遗传图谱与表型数据相结合进行了QTL定位分析。影响瘦肉率等活体估计性状的QTL位于2号染色体的60 - 65 cM处，而背最长肌高度和大理石花纹的QTL分别位于20 cM和55 cM处。其中，估计瘦肉率的QTL在染色体水平上显著（P < 0.01），占表型变异的21.55%。背最长肌高度和大理石花纹的QTL分别占表型变异的10.12%和10.97%。活体估计性状的加性效应和显性效应呈相反趋势，而背最长肌高度的QTL其加性效应和显性效应呈相同趋势，且在大白猪中具有有利等位基因。我们检测到的QTL对表型有较大影响，为分子标记辅助选择和育种奠定了基础。
