Wang Shan-Shan, Zhan Dao-Cheng, Peng Gui-Fu, Pan Hua, Xiao Hong, Zeng Nian-Hua, Huang Jia-Liang, Wang Zhi-Bing
Department of Epidemiology, Medical Institute of Guangzhou Command PLA, Yanling, Guangzhou, People's Republic of China.
Southeast Asian J Trop Med Public Health. 2002 Sep;33(3):551-6.
The genotype of Orientia tsutsugamushi DNA from mites in the Xisa archipelago of China were identified. A natural focus of tsutsugamushi disease in the archipelago was found. The DNA sequence that codes for the 56 kDa protein of O. tsutsugamushi was amplified by nested polymerase chain reaction (N-PCR). The purified positive products were cloned into a pGEM-T vector and sequenced. The DNA sequence was compared with various sequences on the internet for sequence homology. A 507 bp DNA fragment encoding the 56 kDa protein was amplified from the samples. The sequence homology was 85% (Karp strain), 68% (Gilliam strain), 65% (Kato strain), and 67% (Yonchon strain). Orientia tsutsugamushi is carried by the mites of the Xisa archipelago; the main genotype is the Karp strain.
对中国西沙群岛恙螨体内恙虫病东方体DNA的基因型进行了鉴定。发现该群岛存在恙虫病自然疫源地。通过巢式聚合酶链反应（N-PCR）扩增了恙虫病东方体56 kDa蛋白的编码DNA序列。将纯化的阳性产物克隆到pGEM-T载体中并进行测序。将该DNA序列与互联网上的各种序列进行比对以分析序列同源性。从样本中扩增出了一段编码56 kDa蛋白的507 bp DNA片段。其与Karp株的序列同源性为85%，与Gilliam株的为68%，与Kato株的为65%，与Yonchon株的为67%。西沙群岛的恙螨携带恙虫病东方体；主要基因型为Karp株。
