泰国恙虫病东方体血清型的巢式聚合酶链反应测定及其地理分布

Determination and geographic distribution of Orientia tsutsugamushi serotypes in Thailand by nested polymerase chain reaction.

作者信息

Manosroi Jiradej, Chutipongvivate Salakchit, Auwanit Wattana, Manosroi Aranya

机构信息

Pharmaceutical Cosmetic Raw Materials and Natural Products Research and Development Center (PCRNC), Institute for Science and Technology Research and Development/Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.

出版信息

Diagn Microbiol Infect Dis. 2006 Jul;55(3):185-90. doi: 10.1016/j.diagmicrobio.2006.01.014. Epub 2006 Apr 19.

DOI:10.1016/j.diagmicrobio.2006.01.014

PMID:16626907

Abstract

Clotted blood samples of 240 scrub typhus patients were collected from 8 Regional Medical Sciences Centers in Thailand during 1999 to 2002. The serotypes of Orientia tsutsugamushi and their geographic distribution were determined. A nested polymerase chain reaction (PCR) was used to identify the serotypes of O. tsutsugamushi. The number of patients with positive results for O. tsutsugamushi was 25.0%. Two serotypes, Karp and Kato, were detected in these samples. No Gilliam serotype was detected from any of the study locations. The PCR products were sequenced using an automated DNA sequencer. The nucleotide sequence of gene encoding 56-kDa protein from these samples showed a high sequence homology with the reference sequence of O. tsutsugamushi Karp and Kato serotypes. O. tsutsugamushi Karp serotype was predominant throughout Thailand with the percentage of 96.8% of the total serotype-positive patients, whereas 3.2% for Kato serotype was observed only in the south. The highest number among the region of Karp serotype-positive patients of 31.6% was found in the northeast.

摘要

1999年至2002年期间，从泰国8个地区医学科学中心收集了240例恙虫病患者的凝血样本。确定了恙虫病东方体的血清型及其地理分布。采用巢式聚合酶链反应（PCR）鉴定恙虫病东方体的血清型。恙虫病东方体检测结果呈阳性的患者比例为25.0%。在这些样本中检测到了两种血清型，即Karp型和Kato型。在任何研究地点均未检测到Gilliam血清型。使用自动DNA测序仪对PCR产物进行测序。这些样本中编码56-kDa蛋白的基因核苷酸序列与恙虫病东方体Karp型和Kato型的参考序列具有高度序列同源性。恙虫病东方体Karp血清型在泰国各地占主导地位，占血清型阳性患者总数的96.8%，而Kato血清型仅在南部观察到，占3.2%。Karp血清型阳性患者比例最高的地区为东北部，达31.6%。