Scarel-Caminaga Raquel M, Trevilatto Paula C, Souza Ana P, Brito Rui B, Line Sergio R P
Department of Morphology, Dental School of Piracicaba, State University of Campinas, Av. Limeira 901, CEP 13414-018, Piracicaba-SP, Brazil.
J Clin Periodontol. 2003 Apr;30(4):341-5. doi: 10.1034/j.1600-051x.2003.00317.x.
Cytokines are key factors that mediate the inflammatory process during periodontal disease. Recent works have shown that the levels of cytokine expression are regulated by genetic polymorphisms, and that these variations can interfere with the progression of disease. The-590 (C-->T) polymorphism of the IL4 gene is associated with high levels of IgE in asthmatic families, and the frequency of the T allele was increased in asthmatic children. The concentration of IgE in gingival tissue was found to be elevated in patients with periodontitis.
In this study the relationship between the-590 (C-->T) polymorphism in the IL4 gene and different levels of chronic periodontal disease was investigated.
DNA was extracted from buccal epithelial cells of 113 unrelated adult individuals with different levels of periodontitis. The PCR-RFLP technique was used to investigate the polymorphism in the promoter of IL4 gene.
No significant differences in the allele and genotype frequencies of the polymorphism were found between control and groups with periodontal disease.
: We conclude that the-590 (C-->T) polymorphism in the IL4 gene is not associated with the susceptibility to chronic periodontal disease.
细胞因子是介导牙周疾病炎症过程的关键因素。近期研究表明，细胞因子表达水平受基因多态性调控，且这些变异可干扰疾病进展。白细胞介素4（IL4）基因-590（C→T）多态性与哮喘家族中高水平的免疫球蛋白E（IgE）相关，且哮喘儿童中T等位基因频率增加。牙周炎患者牙龈组织中IgE浓度升高。
本研究调查IL4基因-590（C→T）多态性与不同程度慢性牙周疾病之间的关系。
从113名患有不同程度牙周炎的无亲缘关系成年个体的颊黏膜上皮细胞中提取DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术研究IL4基因启动子区的多态性。
牙周疾病组与对照组之间，该多态性的等位基因和基因型频率无显著差异。
我们得出结论，IL4基因-590（C→T）多态性与慢性牙周疾病易感性无关。
