Departmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, Brazil.
Mediators Inflamm. 2012;2012:846052. doi: 10.1155/2012/846052. Epub 2012 Dec 6.
Interleukin-17 (IL-17) is a cytokine that induces neutrophil recruitment and the release of inflammatory mediators in several inflammatory conditions; nevertheless, the involvement of IL-17 gene polymorphisms in chronic periodontitis (CP) has not been addressed yet. Our aim was to evaluate the association between periodontal status and the polymorphisms IL-17A G197A and IL-17F C7488T in subjects with CP along with their impact on levels of inflammatory mediators.
Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). IL-17A G197A and IL-17F C7488T polymorphisms were determined using PCR-RFLP. Serum and periodontal tissues were collected and processed for ELISA, myeloperoxidase (MPO), and/or microscopic analysis.
The frequencies of genotypes in the CP group were significantly different from those of HC. Odds ratio indicated that increased risks for CP were associated with the -197A allele, not with the -7488T allele. In addition, the -197A allele was correlated with worse clinical parameters, higher MPO activity, and increased expression of inflammatory mediators (IL-17A and IL-8) than the other genotypes.
These results indicate that the IL-17A -197A allele is associated with increased risk for CP, likely because this genotype relates to the enhanced inflammation in periodontal tissues.
白细胞介素-17（IL-17）是一种细胞因子，可在多种炎症情况下诱导中性粒细胞募集和炎症介质的释放；然而，IL-17 基因多态性与慢性牙周炎（CP）的关系尚未得到解决。我们的目的是评估 CP 患者的牙周状况与 IL-17A G197A 和 IL-17F C7488T 多态性之间的关系及其对炎症介质水平的影响。
从 30 例 CP 患者和 30 例健康对照者（HCs）中获得基因组 DNA。使用 PCR-RFLP 检测 IL-17A G197A 和 IL-17F C7488T 多态性。采集血清和牙周组织，用于 ELISA、髓过氧化物酶（MPO）和/或显微镜分析。
CP 组的基因型频率与 HCs 有显著差异。比值比表明，-197A 等位基因与 CP 的风险增加有关，而-7488T 等位基因则没有。此外，-197A 等位基因与更差的临床参数、更高的 MPO 活性以及炎症介质（IL-17A 和 IL-8）表达增加相关，与其他基因型相比。
这些结果表明，IL-17A-197A 等位基因与 CP 的风险增加有关，可能是因为这种基因型与牙周组织的炎症增强有关。
