Mikaelsdottir Evgenia K, Benediktsdottir Kristrun R, Olafsdottir Kristrun, Arnadottir Thorgerdur, Ragnarsson Gunnar B, Olafsson Karl, Sigurdsson Kristjan, Kristjansdottir Gudny S, Imsland Albert K, Ogmundsdottir Helga M, Rafnar Thorunn
Laboratory of Molecular and Cell Biology, The Icelandic Cancer Society, Skogarhlid 8, 105 Reykjavik, Iceland.
Gynecol Oncol. 2003 Apr;89(1):22-30. doi: 10.1016/s0090-8258(03)00053-2.
Cervical cancer is a disease caused in part by an infection with an oncogenic subtype of human papillomavirus (HPV). In this study we analysed all cervical cancer samples diagnosed in Iceland during two periods, 1958-1960 and 1995-1996, and asked whether significant changes in viral or immunological parameters had occurred over a period that spanned both significant changes in sexual attitude and the implementation of organized screening for cervical cancer.
Samples from 47 patients (46 squamous cell carcinomas (SCC) and 1 adenosquamous carcinoma (ASC)) in the first period and 30 patients (20 SCC, 4 ASC, and 6 adenocarcinomas (AC)) in the later period were analysed for viral subtype and expression of Fas, FasL, MHC class I, p53 and apoptosis.
AC and ASC are proportionately much more common today than 40 years ago (30% vs 2%). The distribution of HPV in cervical cancer is similar in both periods, with HPV16 found in 75% and HPV18 in 13% of cases. Other HPV types found were 31,33,45, and 59. No significant differences were found in the immunological profiles of tumors from the two periods except that a higher fraction of SCC in the later period stained positive for FasL. When SCC are compared with AC/ASC, the latter have less expression of MHC class I, less expression of Fas, and stronger FasL expression.
AC/ASC tumors show some immunological features that suggest that they are more resistant to immune attack than SCC.
宫颈癌部分是由人乳头瘤病毒（HPV）致癌亚型感染所致。在本研究中，我们分析了冰岛在两个时期（1958 - 1960年和1995 - 1996年）诊断的所有宫颈癌样本，探讨在跨越性观念显著变化和宫颈癌有组织筛查实施的这段时期内，病毒或免疫参数是否发生了显著变化。
分析了第一时期47例患者（46例鳞状细胞癌（SCC）和1例腺鳞癌（ASC））以及后期30例患者（20例SCC、4例ASC和6例腺癌（AC））的样本，检测病毒亚型以及Fas、FasL、MHC I类分子、p53的表达和细胞凋亡情况。
如今，AC和ASC的比例比40年前显著增加（30%对2%）。两个时期宫颈癌中HPV的分布相似，75%的病例中发现HPV16，13%的病例中发现HPV18。还发现了其他HPV类型，如31、33、45和59。两个时期肿瘤的免疫特征无显著差异，只是后期SCC中FasL染色阳性的比例更高。当将SCC与AC/ASC比较时，后者MHC I类分子表达较少、Fas表达较少，而FasL表达更强。
AC/ASC肿瘤表现出一些免疫特征，提示它们比SCC对免疫攻击更具抗性。
