Ikenberg H, Sauerbrei W, Schottmüller U, Spitz C, Pfleiderer A
Department of Obstetrics and Gynecology, University of Freiburg, Germany.
Int J Cancer. 1994 Nov 1;59(3):322-6. doi: 10.1002/ijc.2910590306.
Human papillomavirus (HPV) is a main factor in cervical carcinogenesis. However, data on the correlation of HPV with clinical features and the prognosis of cervical carcinoma remain controversial. The HPV status (positivity, type, copy number) in unfixed tissue specimens of 205 primary invasive cervical carcinomas was determined by Southern blot hybridization. A correlation with comprehensive clinical and histopathologic data and long-time survival was evaluated. HPV DNA was detected in 73% of the cases; 83% of the HPV-positive tumors contained HPV 16. HPV 16 was predominant among squamous-cell carcinomas (SCC) (p = 0.05). HPV 16 copy number was higher in keratinizing tumors (p < 0.05), and elevated levels of the SCC antigen were more common in patients positive for HPV 16 (p < 0.03). No association was found between the HPV status and 8 other clinical and histopathologic variables. Multivariate analysis after a median follow-up of 73 months demonstrated longer survival for patients with lower clinical stage (p = 0.001) and keratinizing SCC (p = 0.005). Women with HPV-negative tumors had a higher risk of death (RR 1.51; p = 0.07). HPV analysis does not clearly define biologically distinct sub-sets of cervical carcinoma. This underlines the importance of additional factors in cervical carcinogenesis.
人乳头瘤病毒（HPV）是宫颈癌发生的主要因素。然而，关于HPV与宫颈癌临床特征及预后相关性的数据仍存在争议。通过Southern印迹杂交法测定了205例原发性浸润性宫颈癌未固定组织标本中的HPV状态（阳性、类型、拷贝数）。评估了其与综合临床和组织病理学数据及长期生存的相关性。73%的病例检测到HPV DNA；83%的HPV阳性肿瘤含有HPV 16。HPV 16在鳞状细胞癌（SCC）中占主导地位（p = 0.05）。HPV 16拷贝数在角化性肿瘤中更高（p < 0.05），SCC抗原水平升高在HPV 16阳性患者中更常见（p < 0.03）。未发现HPV状态与其他8个临床和组织病理学变量之间存在关联。中位随访73个月后的多变量分析表明，临床分期较低（p = 0.001）和角化性SCC（p = 0.005）的患者生存期更长。HPV阴性肿瘤的女性死亡风险更高（相对风险1.51；p = 0.07）。HPV分析不能明确界定宫颈癌在生物学上不同的亚组。这突出了宫颈癌发生中其他因素的重要性。
