Cruces M P, Pimentel E, Zimmering S
Departamento de Genetica, Instituto Nacional de Investigaciones Nucleares (ININ), Edo. de Mexico, Mexico city, Mexico.
Mutat Res. 2003 Apr 20;536(1-2):139-44. doi: 10.1016/s1383-5718(03)00043-3.
In Drosophila, 48h-old larvae were pretreated for 24h with chlorophyllin (CHLN) or sucrose and then treated with chromium(VI) oxide (CrO(3)) immediately following completion of the pretreatment period (0-day delay) or delayed 1, 2 or 3 days. The effects were scored in the wing spot test. After delays of 0 and 1 day, clear evidence of a protective effect of CHLN was found. Contrarily, after delays of 2 and 3 days, the results showed a reversal, i.e. CHLN-related events appeared more frequently than those in the sucrose control suggesting a promoting effect. It would appear prudent that CHLN be tested in a variety of situations in any given organism before decisions are reached regarding its inhibitor/promoter effects.
在果蝇中，48小时龄的幼虫用叶绿酸（CHLN）或蔗糖预处理24小时，然后在预处理期结束后立即（0天延迟）或延迟1、2或3天用三氧化铬（CrO₃）处理。在翅斑试验中对效果进行评分。在0天和1天延迟后，发现了CHLN具有保护作用的明确证据。相反，在2天和3天延迟后，结果显示出逆转，即与CHLN相关的事件比蔗糖对照组中出现得更频繁，表明有促进作用。在就CHLN的抑制剂/促进剂作用做出决定之前，在任何给定生物体的各种情况下对其进行测试似乎是谨慎的做法。
