Riesing Martin J, Kruckenhauser Luise, Gamauf Anita, Haring Elisabeth
Museum of Natural History Vienna, Burgring 7, A-1014, Vienna, Austria.
Mol Phylogenet Evol. 2003 May;27(2):328-42. doi: 10.1016/s1055-7903(02)00450-5.
DNA sequences of the mitochondrial nd6 gene and the non-repetitive part of the pseudo-control region (PsiCR) were isolated from 101 individuals to analyze the phylogenetic relationships among all buzzards of the genus Buteo and other buteonine genera. Comparisons of the two marker sequences indicate that the PsiCR evolved two times faster than the nd6 gene. The PsiCR proved to be an efficient, neutral genetic marker sequence for phylogenetic analyses at the intrageneric level, especially suitable for analyses based on old tissues, where only short fragments can be obtained. The molecular data set implies a neotropical origin of the genus Buteo. Monophyly of the genus Buteo as currently defined is contradicted due to the positions of Asturina nitida, Geranoaetus melanoleucus, Buteo magnirostris, and Buteo leucorrhous. These findings suggest several taxonomic consequences. A. nitida and G. melanoleucus should be included into the genus Buteo. Moreover, B. leucorrhous should be transferred into the genus Percnohierax (which clusters with Parabuteo), and B. magnirostris into the genus Rupornis. According to this classification of the genus Buteo, the basal lineage of the genus is formed by a clade containing Buteo polyosoma, Buteo poecilochrous, and Buteo melanoleucus. The "woodland buteos" form a paraphyletic assemblage with B. magnirostris as a clearly separated lineage basal to the genus Buteo.
从101个个体中分离出线粒体nd6基因和假控制区（PsiCR）非重复部分的DNA序列，以分析鵟属所有鵟与其他鵟亚科属之间的系统发育关系。对这两个标记序列的比较表明，PsiCR的进化速度比nd6基因快两倍。PsiCR被证明是用于属内水平系统发育分析的一种高效、中性的遗传标记序列，特别适用于基于旧组织的分析，因为在旧组织中只能获得短片段。分子数据集表明鵟属起源于新热带地区。由于黑背鹰、黑腹蛇鹈、大嘴鵟和白腰鵟的位置，目前所定义的鵟属的单系性受到了质疑。这些发现表明了几个分类学上的结果。黑背鹰和黑腹蛇鹈应归入鵟属。此外，白腰鵟应转入与凤头鹰属聚在一起的强脚鹰属，大嘴鵟应转入燕尾鹰属。根据鵟属的这种分类，该属的基部谱系由一个包含纯色鵟、杂色鵟和黑腹鵟的分支形成。“林地鵟”形成了一个并系类群，大嘴鵟是鵟属基部一个明显分开的谱系。
