[Comparative morphology of the livers in amphibians].

Amphibians are divided into three orders: Gymnophiona, Urodela and Anura. We studied on the correlation between the liver structures in the three orders of amphibians by histological technique. Livers of thirty species of amphibian were fixed by perfusion with paraformaldehyde, and observed by light microscopy in special staining for elastic fibers. The cytoskeletal components of the hepatic stellate cells and myofibroblasts were identified by immunohistochemistry for glial fibrillary acidic protein and a-smooth muscle actin. Among the three orders of amphibians, Gymnophiona and Urodela were characterized the development of lymph vessels in liver capsules and Glisson's sheath. The present study indicates that there are differences in the pattern of hepatic histological components in the amphibians examined at this time. We discussed their diverged mode of living that can be categorized into three groups: aquatic, aqua-terrestrial and fully terrestrial group.