Zini Cláudia A, Zanin Kelen D, Christensen Eva, Caramão Elina B, Pawliszyn Janusz
Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
J Agric Food Chem. 2003 Apr 23;51(9):2679-86. doi: 10.1021/jf026047g.
Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography and ion-trap mass spectrometry has been used to identify biogenic volatile organic compounds present in the headspace of chopped leaves of Eucalyptus (E.) dunnii, E. citriodora, and E. saligna. A simple HS-SPME method entailing 30 min of extraction at 30 degrees C was developed for this purpose. Thirty compounds were identified in the headspace of 60 juvenile chopped Eucalyptus leaves, and another 30 were tentatively identified. The presence of compounds such as (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMNT), (E,E)-alpha-farnesene, (E,E,E)-3,7,11,15-tetramethyl-1,3,6,10,14-hexadecapentaene (TMHP), beta-caryophyllene, alpha-humulene, germacrene D, and beta-cubebene in the headspace of the leaves but not in the essential oils from the same Eucalyptus trees and information about the infochemical roles of some of these compounds in other living plant systems suggest they might play a bioactive role in Eucalyptus leaves.
顶空固相微萃取（HS-SPME）结合气相色谱和离子阱质谱已被用于鉴定邓恩桉、柠檬桉和柳桉切碎叶片顶空中存在的生物源挥发性有机化合物。为此开发了一种简单的HS-SPME方法，即在30℃下萃取30分钟。在60片幼年桉树叶切碎后的顶空中鉴定出30种化合物，另有30种化合物初步鉴定。叶片顶空中存在（E）-4,8-二甲基-1,3,7-壬三烯（DMNT）、（E,E）-4,8,12-三甲基-1,3,7,11-十三碳四烯（TMNT）、（E,E）-α-法尼烯、（E,E,E）-3,7,11,15-四甲基-1,3,6,10,14-十六碳五烯（TMHP）、β-石竹烯、α-葎草烯、杜松烯D和β-荜澄茄烯等化合物，但在同一桉树的精油中不存在，并且关于其中一些化合物在其他活植物系统中的信息化学作用表明它们可能在桉树叶中发挥生物活性作用。
