Renda Gülin, Özel Arzu, Barut Burak, Korkmaz Büşra, Yayli Nurettin
Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Iran J Pharm Res. 2019 Fall;18(4):1831-1842. doi: 10.22037/ijpr.2019.1100802.
The volatile organic compounds (VOCs) of two species ( Miller and L.) obtained by hydrodistillation (HD) and solid phase microextraction (SPME) techniques were identified by GC-FID/MS. The major compounds identified in the SPME extracts were limonene (43.4%) in ocimene and ()--ocimene (34.3% and 32.4%) in whereas, the essential oils of and were rich with -terpinene (22.4%) and phytol (30.7%), respectively. In addition, acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), tyrosinase, -glucosidase enzyme inhibitory, and radical scavenging activities (DPPH) of chloroform, ethyl acetate, methanol, and water extracts, and also essential oils obtained from and were investigated. The tyrosinase activity was studied at the doses of 25 µg/mL, 50 µg/mL and 100 µg/mL. Tyrosinase inhibition percentage was observed to increase by dose and methanol extracts of the both species were found to have the highest activity. Essential oils of the both species were found to have significant acetylcholinesterase and butyrylcholinesterase inhibition activities. -Glucosidase enzyme inhibition of the ethyl acetate and water extracts of was determined as 80.11 ± 4.07% and 80.32 ± 3.47% at the 100 µg/mL concentration, respectively. Essential oils, chloroform, ethyl acetate, methanol, and water extracts were determined to have moderate DPPH radical scavenging activities.
采用气相色谱-火焰离子化检测器/质谱联用仪（GC-FID/MS）对通过水蒸馏（HD）和固相微萃取（SPME）技术获得的两种植物（Miller和L.）的挥发性有机化合物（VOCs）进行了鉴定。在固相微萃取提取物中鉴定出的主要化合物，在[具体植物1]中为柠檬烯（43.4%）、罗勒烯和（）-（-）-罗勒烯（分别为34.3%和32.4%），而在[具体植物2]和[具体植物3]的精油中，分别富含对伞花烃（22.4%）和叶绿醇（30.7%）。此外，还研究了[具体植物2]和[具体植物3]的氯仿、乙酸乙酯、甲醇和水提取物以及精油的乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BuChE）、酪氨酸酶、α-葡萄糖苷酶抑制活性和自由基清除活性（DPPH）。在25μg/mL、50μg/mL和100μg/mL的剂量下研究了酪氨酸酶活性。观察到酪氨酸酶抑制率随剂量增加而升高，且两种植物的甲醇提取物具有最高活性。发现两种植物的精油均具有显著的乙酰胆碱酯酶和丁酰胆碱酯酶抑制活性。在100μg/mL浓度下，[具体植物2]的乙酸乙酯和水提取物对α-葡萄糖苷酶的抑制率分别为80.11±4.07%和80.32±3.47%。测定精油、氯仿、乙酸乙酯、甲醇和水提取物具有中等的DPPH自由基清除活性。
