Chaudhuri Asok, Rodriguez Marilis, Zbrzezna Valerie, Luo Hong, Pogo A Oscar, Banerjee Deben
Laboratory of Cell Biology, The New York Blood Center, 310 East 67th Street, New York, NY 10021, USA.
Cytokine. 2003 Feb 7;21(3):137-48. doi: 10.1016/s1043-4666(03)00033-4.
Duffy Blood Group protein is a glycoprotein with seven transmembrane domains that binds to C-X-C and C-C chemokines. The antigen is constitutively expressed in endothelial and epithelial cells of several nonerythroid tissues and in Purkinje cells of the cerebellum. We studied the effect of proinflammatory cytokines on Duffy gene expression in endothelial cells from human umbilical vein (HUVEC) and human pulmonary arteries (HPAEC). Also, we studied the effect of inflammatory agents like bacterial lipopolysaccharide (LPS) on Duffy gene induction in mouse. Reverse transcription-PCR and mRNA blot analyses showed that Duffy mRNA was present in these cells in negligible amounts. However, treatment with tumor necrosis factor-alpha for 6-24h resulted in a 5 to 8-fold increase in Duffy mRNA. On the other hand, treatment with interleukin-1 (IL-1), IL-6 or LPS did not have any effect. Fluorescence microscopy and fluorescence activated cell sorting showed greater expression of Duffy protein in treated cells correlating the increase in mRNA synthesis with an increase in antigen production. In mice, Duffy gene was induced in lungs and brain with LPS treatment indicating that the induction is a physiological event. Vascular endothelial cells may induce Duffy protein to regulate leukocytes and/or chemokine trafficking.
达菲血型蛋白是一种具有七个跨膜结构域的糖蛋白，可与C-X-C和C-C趋化因子结合。该抗原在几种非红细胞组织的内皮细胞和上皮细胞以及小脑的浦肯野细胞中组成性表达。我们研究了促炎细胞因子对人脐静脉内皮细胞（HUVEC）和人肺动脉内皮细胞（HPAEC）中达菲基因表达的影响。此外，我们还研究了细菌脂多糖（LPS）等炎症因子对小鼠达菲基因诱导的影响。逆转录-聚合酶链反应（RT-PCR）和mRNA印迹分析表明，这些细胞中达菲mRNA的含量极少。然而，用肿瘤坏死因子-α处理6-24小时后，达菲mRNA增加了5至8倍。另一方面，用白细胞介素-1（IL-1）、IL-6或LPS处理没有任何效果。荧光显微镜和荧光激活细胞分选显示，处理后的细胞中达菲蛋白表达增加，这表明mRNA合成的增加与抗原产生的增加相关。在小鼠中，LPS处理可诱导肺和脑中的达菲基因，这表明这种诱导是一种生理事件。血管内皮细胞可能诱导达菲蛋白来调节白细胞和/或趋化因子的运输。
