Rousseau-Merck M F, Koczan D, Legrand I, Möller S, Autran S, Thiesen H-J
Inserm U509, Institut Curie, Paris, France.
Cytogenet Genome Res. 2002;98(2-3):147-53. doi: 10.1159/000069802.
The chromosome locations of 368 human Kruppel-type zinc finger (ZNF) PAC clones were physically mapped by FISH to human chromosomes in support of recent efforts of assigning KOX cDNAs (KOX1-KOX32) to zinc finger gene clusters. Recent mapping results were validated and confirmed by sequence comparisons to zinc finger gene sequences automatically annotated in EnsEMBL. In toto, 799 Kruppel-type zinc finger genes have been annotated in EnsEMBL of which 290 genes are found to encode KRAB domains. Sequence homologies of the zinc finger domains were used to establish phylogenic trees of KOX zinc finger genes as well as of all KRAB containing human zinc finger and KOX genes documenting the evolution of KRAB zinc finger genes late in primate evolution. A list of 368 assigned ZNF PAC clones is available under http://www.pzr.uni-rostock.de/supplements.
通过荧光原位杂交（FISH）将368个人类克鲁ppel型锌指（ZNF）PAC克隆的染色体位置物理定位到人类染色体上，以支持最近将KOX cDNA（KOX1-KOX32）分配到锌指基因簇的工作。通过与EnsEMBL中自动注释的锌指基因序列进行序列比较，验证并确认了最近的定位结果。总体而言，EnsEMBL中已注释了799个克鲁ppel型锌指基因，其中290个基因被发现编码KRAB结构域。利用锌指结构域的序列同源性建立了KOX锌指基因以及所有含KRAB的人类锌指和KOX基因的系统发育树，记录了KRAB锌指基因在灵长类进化后期的进化过程。可在http://www.pzr.uni-rostock.de/supplements获取368个已分配的ZNF PAC克隆列表。
