Varbanets L D, Vasil'ev V N, Brovarskaia O S
Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, 252143 Ukraine.
Mikrobiologiia. 2003 Jan-Feb;72(1):19-25.
Lipopolysaccharides (LPSs) from four strains of Ralstonia solanacearum belonging to biovar I (ICMP 6524, 8115, 5712, and 8169) were isolated and investigated. The structural components of the LPS molecule, such as lipid A, the core oligosaccharide, and O-specific polysaccharide (O-PS), were obtained after mild acid hydrolysis of the LPS preparations. In lipid A from all the LPS samples studied, 3-hydroxyhexadecanoic, 2-hydroxyhexadecanoic, tetradecanoic, and hexadecanoic fatty acids prevailed. The dominant monosaccharides of the core oligosaccharides of all of the strains studied were rhamnose, glucose, glucosamine, 2-keto-3-deoxyoctulosonic acid, and heptose. However, individual strains varied in the content of galactose, ribose, xylose, and arabinose. Three types of the O-PS structure were established, which differed in their configuration (alpha or beta), as well as in the type of the bond between glucosamine and rhamnose residues (1-->2 or 1-->3).
对属于生物变种I的四株青枯雷尔氏菌（ICMP 6524、8115、5712和8169）的脂多糖（LPS）进行了分离和研究。LPS分子的结构成分，如脂质A、核心寡糖和O-特异性多糖（O-PS），在LPS制剂经温和酸水解后获得。在所研究的所有LPS样品的脂质A中，以3-羟基十六烷酸、2-羟基十六烷酸、十四烷酸和十六烷酸为主。在所研究的所有菌株的核心寡糖中，主要的单糖是鼠李糖、葡萄糖、氨基葡萄糖、2-酮-3-脱氧辛酸和庚糖。然而，各菌株在半乳糖、核糖、木糖和阿拉伯糖的含量上有所不同。确定了三种类型的O-PS结构，它们在构型（α或β）以及氨基葡萄糖和鼠李糖残基之间的键类型（1→2或1→3）上有所不同。
