Park In-Kyu, Yang Jun, Jeong Hwan-Jeong, Bom Hee-Seung, Harada Ichiro, Akaike Toshihiro, Kim Su-Il, Cho Chong-Su
School of Agricultural Biotechnology, Seoul National University, 103 Serdun-dong, Kwonsun-gu, Suwon 441-744, South Korea.
Biomaterials. 2003 Jun;24(13):2331-7. doi: 10.1016/s0142-9612(03)00108-x.
Galactose moiety as the hepatocyte anchorage was covalently coupled with chitosan for the development of synthetic extracellular matrix. Hepatocytes adhesion to galactosylated chitosan (GC)-coated polystyrene (PS) dish became as high as 94.7% after 2 h incubation whereas the hepatocytes adhesion to chitosan-coated PS dish was 69.1%, indication of galactose-specific recognition between GC molecules and asialoglycoprotein receptors of hepatocytes. The DNA synthesis of the hepatocytes adhered to GC-coated dish was increased in the presence of epidermal growth factor (EGF) at low concentration of GC (0.05 microg/ml) whereas the DNA synthesis of the hepatocytes adhered to GC-coated dish was decreased in the presence of EGF at high concentration of GC (5 microg/ml). The spreading shapes of the hepatocytes adhered to the surface in the presence of EGF at low concentration of GC (0.05 microg/ml) were enhanced than in the absence of EGF. The hepatocytes adhered to the surface at high concentration of GC (5 microg/ml) showed round shapes and exhibited many spheroid formation after 24 h in the presence of EGF.
将半乳糖部分作为肝细胞锚定物与壳聚糖共价偶联，用于合成细胞外基质的研发。在孵育2小时后，肝细胞在半乳糖基化壳聚糖（GC）包被的聚苯乙烯（PS）培养皿上的黏附率高达94.7%，而在壳聚糖包被的PS培养皿上的黏附率为69.1%，这表明GC分子与肝细胞的去唾液酸糖蛋白受体之间存在半乳糖特异性识别。在低浓度GC（0.05微克/毫升）存在表皮生长因子（EGF）的情况下，黏附于GC包被培养皿上的肝细胞的DNA合成增加；而在高浓度GC（5微克/毫升）存在EGF的情况下，黏附于GC包被培养皿上的肝细胞的DNA合成减少。在低浓度GC（0.05微克/毫升）存在EGF的情况下，黏附于表面的肝细胞的铺展形态比不存在EGF时有所增强。在高浓度GC（5微克/毫升）存在EGF的情况下，黏附于表面的肝细胞呈圆形，在24小时后出现许多球体形成。
