Saha Badal C, Nakamura Lawrence K
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, Illinois 61604, USA.
Biotechnol Bioeng. 2003 Jun 30;82(7):864-71. doi: 10.1002/bit.10638.
Lactobacillus intermedius B-3693 was selected as a good producer of mannitol from fructose after screening 72 bacterial strains. The bacterium produced mannitol, lactic acid, and acetic acid from fructose in pH-controlled batch fermentation. Typical yields of mannitol, lactic acid, and acetic acid from 250 g/L fructose were 0.70, 0.16, and 0.12 g, respectively per g of fructose. The fermentation time was greatly dependent on fructose concentration but the product yields were not dependent on fructose level. Fed-batch fermentation decreased the time of maximum mannitol production from fructose (300 g/L) from 136 to 92 h. One-third of fructose could be replaced with glucose, maltose, galactose, mannose, raffinose, or starch with glucoamylase (simultaneous saccharification and fermentation), and two-thirds of fructose could be replaced with sucrose. L. intermedius B-3693 did not co-utilize lactose, cellobiose, glycerol, or xylose with fructose. It produced lactic acid and ethanol but no acetic acid from glucose. The bacterium produced 21.3 +/- 0.6 g lactic acid, 10.5 +/- 0.3 g acetic acid, and 4.7 +/- 0.0 g ethanol per L of fermentation broth from dilute acid (15% solids, 0.5% H(2)SO(4), 121 degrees C, 1 h) pretreated enzyme (cellulase, beta-glucosidase) saccharified corn fiber hydrolyzate.
在对72株细菌进行筛选后，中间型乳酸杆菌B-3693被选为从果糖高效生产甘露醇的菌株。在pH控制的分批发酵中，该细菌可利用果糖产生甘露醇、乳酸和乙酸。以250 g/L果糖为底物时，甘露醇、乳酸和乙酸的典型产量分别为每克果糖0.70 g、0.16 g和0.12 g。发酵时间很大程度上取决于果糖浓度，但产物产量并不依赖于果糖水平。补料分批发酵将从300 g/L果糖生产甘露醇的最长时间从136小时缩短至92小时。三分之一的果糖可用葡萄糖、麦芽糖、半乳糖、甘露糖、棉子糖或淀粉（通过糖化酶进行同步糖化发酵）替代，三分之二的果糖可用蔗糖替代。中间型乳酸杆菌B-3693不能与果糖共同利用乳糖、纤维二糖、甘油或木糖。它利用葡萄糖产生乳酸和乙醇，但不产生乙酸。该细菌可从稀酸（15%固形物、0.5% H₂SO₄、121℃、1小时）预处理后经酶（纤维素酶、β-葡萄糖苷酶）糖化的玉米纤维水解产物中，每升发酵液产生21.3±0.6 g乳酸、10.5±0.3 g乙酸和4.7±0.0 g乙醇。
