Gaumer H R, Holm-Pedersen P, Folke L E
Infect Immun. 1976 May;13(5):1347-53. doi: 10.1128/iai.13.5.1347-1353.1976.
The blastogenic response of peripheral blood leukocytes to lipopolysaccharide (LPS) was followed over a short course of experimental gingivitis, developed in human volunteers who strictly avoided oral hygeine procedures for periods up to 9 days. Eleven young males initially received thorough dental prophylaxes and supervised oral hygeine until they acquired optimal gingival health. At this point, leukocytes (5 X 10(5)) incubated with 1.5 to 25 mug of LPS in serum-free media showed no response as measured by tritiated thymidine uptake. Coincubation of cells with LPS and phytohemagglutinin (PHA), however, caused synergistic enhancement of blastogenesis in every LPS-PHA dose combination tried. With progressive accumulation of dental plaque and the concomitant development of gingival inflammation, this synergistic response was lost and replaced, proportionately, by a direct response to LPS. The leukocyte response to PHA was marginally enhanced with gingivitis.
在人类志愿者中开展了一项短期实验性牙龈炎研究，这些志愿者严格避免口腔卫生措施长达9天，在此期间观察外周血白细胞对脂多糖（LPS）的增殖反应。11名年轻男性最初接受了全面的牙齿预防治疗并接受监督口腔卫生护理，直至达到最佳牙龈健康状态。此时，在无血清培养基中与1.5至25微克LPS一起孵育的白细胞（5×10⁵），通过氚标记胸腺嘧啶核苷摄取测量显示无反应。然而，细胞与LPS和植物血凝素（PHA）共同孵育，在尝试的每种LPS - PHA剂量组合中均导致增殖的协同增强。随着牙菌斑的逐渐积累以及随之而来的牙龈炎症发展，这种协同反应消失，并相应地被对LPS的直接反应所取代。牙龈炎时白细胞对PHA的反应略有增强。
