Schmidtke J R, Najarian J S
J Immunol. 1975 Feb;114(2 pt 2):742-6.
Mixtures of phytohemagglutinin (PHA) or Concanavalin A (Con A) and lipopolysaccharide (LPS) with human peripheral blood lymphocytes (PBL) resulted in a synergistic effect on DNA synthesis as measured by increased 3H-thymidine uptake. These data were compared to the mitogenic response of human PBL to PHA, Con A, or LPS alone. Various combinations of PHA and Con A usually suppressed DNA synthesis in human PBL. Titration experiments revealed that increasing amounts of LPS, in the presence of a constant amount of Con A, resulted in corresponding increases in the synergistic response of human PBL. Conversely, increasing amounts of Con A, in the presence of a constant amount of LPA did not lead to increases in the amount of synergism. The results are discussed in terms of the participation of additional cells in the synergistic response of human PBL.
将植物血凝素（PHA）或刀豆球蛋白A（Con A）与脂多糖（LPS）的混合物与人外周血淋巴细胞（PBL）一起培养，通过增加³H-胸腺嘧啶核苷摄取量来衡量，结果显示对DNA合成有协同作用。这些数据与人类PBL单独对PHA、Con A或LPS的促有丝分裂反应进行了比较。PHA和Con A的各种组合通常会抑制人类PBL中的DNA合成。滴定实验表明，在Con A量恒定的情况下，LPS量增加会导致人类PBL协同反应相应增加。相反，在LPA量恒定的情况下，Con A量增加并不会导致协同作用量增加。本文根据其他细胞在人类PBL协同反应中的参与情况对结果进行了讨论。
