Yano Tomoyuki, Kishimoto Takashi, Tomaru Utano, Kawarada You, Kato Hiroyuki, Yoshiki Takashi, Ishikura Hiroshi
Department of Molecular Pathology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Pathology. 2003 Feb;35(1):75-8.
To assess the production of a liver-specific protein, albumin, and of a master transcriptional factor, hepatocyte nuclear factor (HNF)-4alpha, in hepatoid adenocarcinoma tissue.
Standard and quantitative RT-PCR, using five cases of hepatoid and three cases of non-hepatoid gastric adenocarcinoma.
Hepatoid adenocarcinomas expressed similarly large amounts of albumin mRNA as those expressed in hepatocellular carcinoma and normal liver tissues. The observed amounts were several hundred times more than those in non-hepatoid adenocarcinoma and normal stomach tissues. HNF-4alpha mRNA was expressed in all stomach samples examined, and the levels of expression did not quantitatively differ between hepatoid and non-hepatoid adenocarcinomas of the stomach.
These results provide further support of a relationship between hepatic transdifferentiation in hepatoid adenocarcinomas and albumin mRNA expression. Furthermore, transdifferentiation to the hepatocytic phenotype in hepatoid adenocarcinoma tissue was not directly associated with HNF-4alpha expression, thus suggesting that transdifferentiation proceeds by a complicated mechanism.
评估肝样腺癌组织中肝脏特异性蛋白白蛋白以及主要转录因子肝细胞核因子（HNF）-4α的产生情况。
采用标准定量逆转录聚合酶链反应（RT-PCR），检测5例肝样胃癌和3例非肝样胃癌。
肝样腺癌表达的白蛋白信使核糖核酸（mRNA）量与肝细胞癌和正常肝组织中表达的量相似。观察到的量比非肝样腺癌和正常胃组织中的量多几百倍。HNF-4α mRNA在所有检测的胃样本中均有表达，且在胃的肝样腺癌和非肝样腺癌之间，其表达水平在数量上没有差异。
这些结果进一步支持了肝样腺癌中的肝转分化与白蛋白mRNA表达之间的关系。此外，肝样腺癌组织向肝细胞表型的转分化与HNF-4α表达没有直接关联，因此表明转分化是通过复杂机制进行的。
